6 October 2010 Meeting

Roll call

• Apologies: Colin Harwood and Jem

Presentation

Introduction

• Mention why do we use this bug and that it is able to live in high pH
• Have to menetion that we all biobrick have been modelled by SBML and copasi

Bacilla Filla slide

• The solution words to be smaller than the logo
• Logo have to be the biggest!
• Solution at the top of the page with a small logo below it then zoom into the logo

Technical review slide

• Show the link between the genes
• Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
• A single shot overview then to the biobricks
• SAY: This is a crack, and here is our bug on the surface of the crack, etc
• Label the pictures!
• Animation for the overview, ie, the background to remain the same

Swarming slide

• Change the flagella of the bug
• Zoom into the plates

Subtilin slide

• Show subtilin only when the bug is at the end of the crack
• Fill up the crack with bugs
• Describle the subtilin signalling system
• Change the pveg promoter to the oxygen sensitive promoter

Subtilin immunity slide

• SAY: Subtilin immunity ablready in the registry, that is why we use it
• Mention what the individual CDS do
• Green tick for those that we did and a red cross for those that we did not

Workflow slide

• Change the word interesting to valuable to all synthetic biologist
• Cut down on the script
• Have to make it fit to the presentation
• Integration of lots of data that is why we use e-science

YneA slide

• Put filamentous cells into the pictures
• Metabolic pathway picture and link it

Glue slide

• The iGEM plates to go onto it

Kill switch

• Can bin it
• Move to the ethic page

Ethic slide

• Move it to the front after the introduction and also mention in the summary
• What is the danger and how we make it safe

Others

• Show that the bug is able to grow in concrete

Urease slide

• Have to show that calcium carbonate is filling up the cracks
• Have to say that we are better than the rest
• Highlight the advantage of ours and the disadvantage of others
• Mention SR1

Lab feedback

Filamentous cells

• Redo the microscopic
• Correlate between the expression of GFP and the length

SR1

• We have colonies

Scanning electron microscopy

• The concrete samples are already with the EM unit

Agenda

• To reedit the agenda

Action points

• Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
• Everyone to do up the presentation slides first.

Next meeting

Wed 4pm.

• Chair: Steven
• Computer: Alan
• Minutes: Deena