Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03

From 2010.igem.org

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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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===2：DNA extraction===
===2：DNA extraction===

Revision as of 11:52, 19 October 2010


Contents

2010/10/03

1:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2

2：DNA extraction

＜Member＞
mio

＜Sample＞
PCR products

  • 4②
  • 4⑤

＜Protocol＞
See Protocol 4


3:Electrophoresis

<Member>
nito

<Sample>
All PCR products(10/3)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-03 17hr 43min.JPG

4：DNA extraction

＜Member＞
mio,mizuki,nito

＜Sample＞
PCR products

  • 1
  • 3
  • 4
  • 5
  • 8
  • 10
  • 4-5
  • 7-8-10
  • 2
  • 6

Digestion products

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 7-8-10

＜Protocol＞
See Protocol 4


5：DNA Ligation

＜Member＞
mio,mizuki

＜Sample＞

  • 1-2
  • 4-5
  • 6A-7
  • 7-8-10

(after digestion)

＜Protocol＞
See Protocol 10


6:Transformation

<Member>
nito

<Sample>

  • 7-8-10
  • JM109

<Protocol>
See Protocol 7


7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • 1
  • 2
  • 3
  • 4
  • 6
  • 10

・Digest enzyme

  • Avr2
  • Nhe1

＜Protocol＞
See Prptpcpl 9

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