"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17
From 2010.igem.org
(Difference between revisions)
(→1:PCR) |
|||
| Line 7: | Line 7: | ||
<Sample><br />(temperature in annealing) | <Sample><br />(temperature in annealing) | ||
| - | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
| - | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===2：DNA Digestion=== | ===2：DNA Digestion=== | ||
Revision as of 11:28, 19 October 2010
Contents |
2010/09/17
1:PCR
<Member>
mio
<Sample>
(temperature in annealing)
<Protocol>
See Protocol 1
2：DNA Digestion
＜Member＞
mizuki
＜Sample, Materials＞
・PCR productions
- 1 (8/31)
- 2 (9/6)
- 4 (8/31)
- 5L (8/26)
- 7 (8/31)
- 8 (8/31)
・Digest enzyme
- 1,4,8 : AvrⅡ
- 2,7 : NheⅠ
- 5L : SpeⅠ
＜Protocol＞
See Prptpcpl 9
3:Electrophoresis①
<Member>
mio
<Sample>
PCR products (9/17)
- 3
- 6
- 8
<Protocol>
See Protocol 8
<Result>
4:Electrophoresis②
<Member>
mizuki
<Sample>
Digestion products (9/17)
- 1
- 2
- 4
- 5
- 7
- 8
<Protocol>
See Protocol 8
<Result>
5：DNA extraction from gels
＜Member＞
mizuki
＜Sample＞
Digestion products(9/17)
- 4
- 7
- 8
(After electrophoresis)
＜Protocol＞
See Protocol 4
6：DNA Ligation
＜Member＞
mizuki
＜Sample＞
- 7(9/17)
- 8(9/17)
(after digestion)
＜Protocol＞
See Protocol 10
