Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/25

From 2010.igem.org

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===1:DNA extraction from gels===
===1:DNA extraction from gels===
<Member><br />
<Member><br />

Revision as of 13:30, 29 September 2010


Contents

2010/09/25

1:DNA extraction from gels

<Member>
mizuki

<Sample>
Digestion products

  • 2
  • 4
  • 5
  • 8

(After electrophoresis)

<Protocol>
See Protocol 4


2:DNA Ligation

<Member>
mizuki

<Sample>

  • 4
  • 5

(after digestion)

<Protocol>
See Protocol 3


3:PCR

<Member>
mizuki

<Sample>(temperature in annealing)

  • 5
  • 7
  • 9
  • 10

<Protocol>
See Protocol 1


4:DNA extraction

<Member>
mizuki

<Sample>

  • 7-8 (9/17)

<Protocol>
See Protocol 4


5:Electrophoresis

<Member>
mizuki

<Sample>
PCR products (9/25)

  • 5
  • 7
  • 9
  • 10

Ligation products (9/25)

  • 4-5

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-25 17hr 28min.JPG

6:DNA extraction

<Member>
mizuki

<Sample>
PCR products

<Protocol>
See Protocol 4


7:DNA Digestion

<Member>
mizuki

<Sample, Materials>

  • 7-8 (9/6)
  • 10 (9/25)

<Protocol>
See Prptpcpl 9