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Team:Newcastle/Meetings/15 September 2010
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==Roll calls== | ==Roll calls== | ||
| - | *Apologies: Phil and Deena | + | * Apologies: Phil and Deena |
| - | *Absence: Colin Harwood, Colin Davie, Da Ye, Wendy | + | * Absence: Colin Harwood, Colin Davie, Da Ye, Wendy |
* Concrete has been made, set in tubes. | * Concrete has been made, set in tubes. | ||
| Line 12: | Line 12: | ||
==Lab feedback== | ==Lab feedback== | ||
* pMutin4 strain transformation is not going very well. Don't just sit and wait to see if ''Bacillus'' transformation worked, do another transformation on the assumption that it hasn't. | * pMutin4 strain transformation is not going very well. Don't just sit and wait to see if ''Bacillus'' transformation worked, do another transformation on the assumption that it hasn't. | ||
| + | * Do chromosomal preps from both strains (168 and 168 with pMutin4), transform with the chromosome from the opposite strain. | ||
* As an alternative approach, Jem's LacI plasmid is here, details will be given on a sheet. | * As an alternative approach, Jem's LacI plasmid is here, details will be given on a sheet. | ||
| - | * | + | |
| + | * SR1 transformed. Check that it is coming in pSB1C3 too. | ||
| + | * Hyperspank | ||
| + | * fluoresence reader and microscope | ||
| + | * Fluostar (Phil Aldridge/Richard?) | ||
| + | * Transformations of LacI E. coli strain today | ||
| + | |||
| + | * Hyperspankoid in pSB1C3 | ||
| + | * Arabinose repressor in pSB1AK3. Has to go into BS. | ||
| + | |||
| + | ==Concrete== | ||
| + | * Try | ||
| + | * Need to make the levans plates with "iGEM" letters | ||
| + | * Try to stick our concrete sticks together | ||
| + | |||
| + | ==Modelling== | ||
| + | Flux balance analysis on wiki by next week | ||
==Action points== | ==Action points== | ||
Revision as of 09:02, 15 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood, Colin Davie, Da Ye, Wendy
- Concrete has been made, set in tubes.
- We went to the EM facility, won't be a problem to take photos of our concrete.
- Wiki design should be ready soon (Harsh's graphic designer friend).
Lab feedback
- pMutin4 strain transformation is not going very well. Don't just sit and wait to see if Bacillus transformation worked, do another transformation on the assumption that it hasn't.
- Do chromosomal preps from both strains (168 and 168 with pMutin4), transform with the chromosome from the opposite strain.
- As an alternative approach, Jem's LacI plasmid is here, details will be given on a sheet.
- SR1 transformed. Check that it is coming in pSB1C3 too.
- Hyperspank
- fluoresence reader and microscope
- Fluostar (Phil Aldridge/Richard?)
- Transformations of LacI E. coli strain today
- Hyperspankoid in pSB1C3
- Arabinose repressor in pSB1AK3. Has to go into BS.
Concrete
- Try
- Need to make the levans plates with "iGEM" letters
- Try to stick our concrete sticks together
Modelling
Flux balance analysis on wiki by next week
Action points
- Neil and Jen - To register the team.
- Alan - To email Jen about the IPTG plasmid and the letters for the sucrose plates.
- Harsh - To crack the concrete into smaller pieces.
- Wendy - To talk to the EM people.
Next meeting
- Chair: Jannetta, Minutes: Steven, Computer: Alan
- Wednesday morning, 9am, CBCB.
   
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