"
Team:TU Delft/Modeling/MFA
From 2010.igem.org
Lbergwerff (Talk | contribs) (→Metabolic Flux Analysis) |
Lbergwerff (Talk | contribs) |
||
| Line 6: | Line 6: | ||
For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified ''E. coli'' metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. ''(link to pages with assumptions)'' The resulting network is the standard network used for adding additional pathways. | For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified ''E. coli'' metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. ''(link to pages with assumptions)'' The resulting network is the standard network used for adding additional pathways. | ||
| + | |||
*implement interactive tool with metabolic network for different pathways here | *implement interactive tool with metabolic network for different pathways here | ||
| + | |||
Implemented pathways | Implemented pathways | ||
| + | *[[Team:TU_Delft/Modeling/MFA/NO3|NO3 as electron acceptor]] | ||
| + | *[[Team:TU_Delft/Modeling/MFA/PHB|PHB production]] | ||
| + | *[[Team:TU_Delft/Modeling/MFA/isoprene|isoprene production]] | ||
| + | *[[Team:TU_Delft/Modeling/MFA/H2|H2 production]] | ||
Revision as of 09:05, 13 September 2010
Metabolic Flux Analysis
A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. With this analysis it is also possible to find whether a system is not viable because of a co-factor imbalance. Lastly, several product pathways were introduced to E. coli with hydrocarbon degradation to see what maximal theoretical yields on hydrocarbons are.
How a metabolic flux analysis works, is explained here.
For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified E. coli metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. (link to pages with assumptions) The resulting network is the standard network used for adding additional pathways.
- implement interactive tool with metabolic network for different pathways here
Implemented pathways
refer to detailed results page here
Go back to previous page here
Click here if you don't like MFA
