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Team:Freiburg Bioware/NoteBook/Labjournal/March
From 2010.igem.org
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[[Media:AAV_alignment.pdf]] | [[Media:AAV_alignment.pdf]] | ||
<br> | <br> | ||
| - | + | The alignment showed significant differences in the sequences of several AAV genomes, | |
resulting in frame shifts. | resulting in frame shifts. | ||
| - | + | We decided to rely on the Stratagene sequence | |
| - | + | ||
'''To do:''' | '''To do:''' | ||
| - | *download and compare | + | *download and compare further AAV serotypes. (In Geneious) |
*compare literature | *compare literature | ||
*find out which possibilities are given to modify the Capsid | *find out which possibilities are given to modify the Capsid | ||
Revision as of 14:37, 14 September 2010
1. Labday 31.03.2010
Sequence analysis
Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)
Theoretical cloning
Sequence analysis (Alignement) of the Stratagene Vector with
- AAV2
- AAV5
- AAV7
- AAV8
result:
Media:AAV_alignment.pdf
The alignment showed significant differences in the sequences of several AAV genomes,
resulting in frame shifts.
We decided to rely on the Stratagene sequence
To do:
- download and compare further AAV serotypes. (In Geneious)
- compare literature
- find out which possibilities are given to modify the Capsid
