Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/26

From 2010.igem.org

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==PCR==
 
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===member===
 
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same above
 
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===Materials===
 
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*2×PCR buffer  25×4μl
 
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*2mM dNTP  10×4μl
 
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*10mM primer(sense)bcsA,B,C and D 2.5μl each
 
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*10mM primer(antisense)bcsA,B,C and D 2.5μl each
 
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*template DNA a little
 
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*Q water 9×4μl
 
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*KOD FX 0.5×4μl
 
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===Procedure===
 
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①mix all materials for 4 tubes.
 
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②elongation
 
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*bcsA,bcsB and bcsC
 
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**94℃ 2min
 
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**98℃ 10sec☆
 
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**55℃ 30sec
 
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**68℃ 4min★
 
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**68℃ 7min
 
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**10℃　∞
 
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*bcsD
 
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**94℃ 2min
 
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**98℃ 10sec☆
 
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**55℃ 30sec
 
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**68℃ 1min★
 
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**68℃ 7min
 
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**10℃　∞
 
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※30cycle ☆ to ★.
 

Revision as of 05:42, 8 September 2010


Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/08/26"