Team:Newcastle/3 September 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
- | + | =''yneA''= | |
- | + | ==Aims== | |
Purify more ''yne''A in pGFPrrnb and check with restriction enzymes. | Purify more ''yne''A in pGFPrrnb and check with restriction enzymes. | ||
- | + | ==Materials and protocols== | |
[[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]] | [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]] | ||
Line 12: | Line 12: | ||
[[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] | [[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] | ||
- | + | ==Results== | |
[[Image:yneAGFPrrnb.jpeg|400px]] | [[Image:yneAGFPrrnb.jpeg|400px]] | ||
- | + | ==Conclusion== | |
The results show that the digest works, there is a band at 541bp corresponding to ''yneA'' and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12. | The results show that the digest works, there is a band at 541bp corresponding to ''yneA'' and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12. | ||
+ | |||
+ | =''roc''F = | ||
+ | |||
+ | ==Aims== | ||
+ | |||
+ | ==Materials and protocols== | ||
+ | |||
+ | [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]] | ||
+ | [[TeamNewcastleNanoDrop_Spectrophotometer| Nanodrop]] | ||
+ | |||
+ | |||
+ | ==Results== | ||
+ | Nanodrop results | ||
+ | |||
+ | # 264.4 ng/ul | ||
+ | # 267.6 ng/ul | ||
+ | # 309.5 ng/ul | ||
+ | # 160.1 ng/ul pure ~ 1.87 | ||
+ | # 218.4 ng/ul | ||
+ | # 233.4 ng/ul | ||
+ | # 258.0 ng/ul | ||
+ | # 201.7 ng/ul | ||
+ | # 237.8 ng/ul | ||
+ | # 201.7 ng/ul | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 10:08, 7 September 2010
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Contents |
yneA
Aims
Purify more yneA in pGFPrrnb and check with restriction enzymes.
Materials and protocols
Plasmid extraction Restriction digest Gel electrophoresis
Results
Conclusion
The results show that the digest works, there is a band at 541bp corresponding to yneA and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
rocF
Aims
Materials and protocols
Results
Nanodrop results
- 264.4 ng/ul
- 267.6 ng/ul
- 309.5 ng/ul
- 160.1 ng/ul pure ~ 1.87
- 218.4 ng/ul
- 233.4 ng/ul
- 258.0 ng/ul
- 201.7 ng/ul
- 237.8 ng/ul
- 201.7 ng/ul