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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/26
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(New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/08/26= ==1：PCR == ＜Member＞<br /> Mariko, mizuki ＜Sample＞<br /> ・E-coli (having BBa_K208017 plasmid) ・E-coli (having BBa_I732901 ...) |
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＜Result＞<br /> | ＜Result＞<br /> | ||
| - | + | [[Image:Matsuura_2010-08-26_16hr_14min.tif]] | |
==5：Transformation with ECOS competent E-coli(NIPPON GENE)== | ==5：Transformation with ECOS competent E-coli(NIPPON GENE)== | ||
Revision as of 09:52, 22 September 2010
Contents |
2010/08/26
1：PCR
＜Member＞
Mariko, mizuki
＜Sample＞
・E-coli (having BBa_K208017 plasmid)
・E-coli (having BBa_I732901 plasmid)
・E-coli(JM109)
＜Protocol＞
See Protocol 2
・Tube(higher temperature/lower temperature in annealing)
2. JM109 (71.5℃/70.0)
4. Promoter(71.5/70.0)
5. RBS~signal (71.5/70.0)
6. lacZ (66.0/63.0)
Total 8 tubes.
2：DNA extraction
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 4
3：DNA concentration measurement
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
| concentration | A320 | A260/A280 | A260/A230 | |
|---|---|---|---|---|
| 2H | 95.5 | 14.84 | 2.011 | 0.054 |
| 2L | 43.0 | -0.025 | 1.792 | 0.203 |
| 4H | 75.0 | 0.044 | 1.852 | 0.346 |
| 4L | 63.0 | -0.004 | 1.881 | 0.565 |
| 5H | 35.0 | 2.96 | 2.000 | 0.160 |
| 5L | 31.0 | -0.020 | 1.873 | 0.071 |
| 6H | 54.5 | -0.078 | 1.946 | 0.215 |
| 6L | 72.0 | 0.501 | 2.257 | 0.029 |
4：Electrophoresis
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
File:Matsuura 2010-08-26 16hr 14min.tif
5：Transformation with ECOS competent E-coli(NIPPON GENE)
＜Member＞
nito
＜Sample＞
・ECOS competent E-coli JM109(NIPPON GENE)
・Parts
- BBa_I13521
- BBa_B0010
- BBa_B0030
- BBa_J23110
- BBa_E0040
＜Protocol＞
SeeProtocol 7
