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Team:Calgary/29 August 2010
From 2010.igem.org
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[[Image:08.29.2010 Chris Colony PCR 1.jpg|thumb|400px|Chris's first gel of the colony PCR. Lanes 1-3 are CpxP2 in AC Vector, Lanes 4-7 are CpxP2 in AK vector, Lanes 8-12 are CpxP1 in AC vector, Lanes 13017 are CpxP3 in AC vector and Lane 18 is CpxP3 in AK vector.]] | [[Image:08.29.2010 Chris Colony PCR 1.jpg|thumb|400px|Chris's first gel of the colony PCR. Lanes 1-3 are CpxP2 in AC Vector, Lanes 4-7 are CpxP2 in AK vector, Lanes 8-12 are CpxP1 in AC vector, Lanes 13017 are CpxP3 in AC vector and Lane 18 is CpxP3 in AK vector.]] | ||
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| + | [[Image:08.29.2010 Chris Colony PCR 2.jpg|thumb|400px|Chris's second gel of the colony PCR. Lanes 1-4 are CpxP3 in AK vector, Lanes 5-9 are ibpAB1 in AC vector, Lanes 10-17 are ibpAB1 in AK vector, and Lane 18 is ibpAB2 in AC vector.]] | ||
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<u>Chris</u> | <u>Chris</u> | ||
Revision as of 15:29, 30 August 2010
Sunday August 29, 2010
Weekends rock!!
Chris
Today, I ran 1.0% gels of the enormous colony PCR that was done yesterday. The gels themselves can be seen to the right with labels. As well, I made overnight cultures of I0500-I13507 construction which will be plasmid prepped tomorrow. Finally, I helped Emily digest and phosphatase MalE so it could be inserted into the AK Biobrick vector.
