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Team:Calgary/28 August 2010
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{{CalgaryNotebookTemplate| | {{CalgaryNotebookTemplate| | ||
Saturday August 28, 2010| | Saturday August 28, 2010| | ||
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| + | Don't we all love weekends? | ||
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<u>Emily</u> | <u>Emily</u> | ||
| - | Today I ran a gel of thr digests that I did yesterday. Unfortuntaley it looks like I was not actually able to get malE into the psB1AK3 Vector. I will go back to the PCR product of this tomorrow. I also ran a gel of my PCR of malESSDel and malE31SSDel. Unfortunately there were no bands, so this will need to be optimized and re-run on Monday. Tonight I also ran a gel of the malE and malE31 PCR Products. This looked | + | Today I ran a gel of thr digests that I did yesterday. Unfortuntaley it looks like I was not actually able to get malE into the psB1AK3 Vector. I will go back to the PCR product of this tomorrow. I also ran a gel of my PCR of malESSDel and malE31SSDel. Unfortunately there were no bands, so this will need to be optimized and re-run on Monday. Tonight I also ran a gel of the malE and malE31 PCR Products. This looked |
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| + | <u>Chris</u> | ||
| + | Today, I set up a 50 reaction colony PCR of the constructs of CpxP into pSB1AC3, CpxP into pSB1AK3, ibpAB into pSB1AK3 and ibpAB into pSB1AC3 to determine whether or not the part had been correctly inserted yesterday. I also started overnight cultures of the part I0500-I13507 which had been constructed previously. I continued writing the paper which is for our wiki about the stress-response circuits that we have chosen. | ||
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Revision as of 06:59, 29 August 2010
Saturday August 28, 2010
Don't we all love weekends?
Emily
Today I ran a gel of thr digests that I did yesterday. Unfortuntaley it looks like I was not actually able to get malE into the psB1AK3 Vector. I will go back to the PCR product of this tomorrow. I also ran a gel of my PCR of malESSDel and malE31SSDel. Unfortunately there were no bands, so this will need to be optimized and re-run on Monday. Tonight I also ran a gel of the malE and malE31 PCR Products. This looked
Chris
Today, I set up a 50 reaction colony PCR of the constructs of CpxP into pSB1AC3, CpxP into pSB1AK3, ibpAB into pSB1AK3 and ibpAB into pSB1AC3 to determine whether or not the part had been correctly inserted yesterday. I also started overnight cultures of the part I0500-I13507 which had been constructed previously. I continued writing the paper which is for our wiki about the stress-response circuits that we have chosen.
