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Team:Calgary/22 August 2010
From 2010.igem.org
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Weekends Rule!! | Weekends Rule!! | ||
| + | [[Image:08.21.2010 ColonyPCRCE..jpg|thumb|400px|Colony PCR agarose gel electrophoresis run on 1.0% at 90V for 45 minutes. Lanes 1 and 2 are K239000-I13507, Lanes 3-6 are K239000-I13504, Lanes 7-16 are I0500-I13507, and Lanes 17 and 18 are Controls with no DNA]] | ||
<u>Chris</u> | <u>Chris</u> | ||
Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow. | Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow. | ||
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Revision as of 17:15, 23 August 2010
Sunday August 22, 2010
Weekends Rule!!
Chris
Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow.
