Team:Newcastle/11 August 2010
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Our aims for today are to run the gel electrophoresis to check whether we have the correct fragement sizes on the four parts that are we amplified [[Team:Newcastle/10 August 2010#Subtilin_Immunity_BioBrick| yesterday]]. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts. | Our aims for today are to run the gel electrophoresis to check whether we have the correct fragement sizes on the four parts that are we amplified [[Team:Newcastle/10 August 2010#Subtilin_Immunity_BioBrick| yesterday]]. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts. | ||
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[[Image:Newcastle_Loaded_Gel_for_Subtilin_Immunity_BioBrick.JPG|right|200px|thumb]] | [[Image:Newcastle_Loaded_Gel_for_Subtilin_Immunity_BioBrick.JPG|right|200px|thumb]] | ||
+ | ==Materials and protocol== | ||
Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]] and the [[Team:Newcastle/Gel_extraction| gel extraction]] protocols. | Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]] and the [[Team:Newcastle/Gel_extraction| gel extraction]] protocols. |
Revision as of 07:08, 12 August 2010
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Contents |
Gibson cloning of the rocF BioBrick
Aim
The aim of this experiment is to assemble the fragments we extracted yesterday in order to construct the rocF BioBrick and clone it into pSB1C3 in one step.
Subtilin Immunity BioBrick
Aims
Our aims for today are to run the gel electrophoresis to check whether we have the correct fragement sizes on the four parts that are we amplified yesterday. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts.
Materials and protocol
Please refer to the gel electrophoresis and the gel extraction protocols.
Results
Figure #: Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick.
- Lane 1: 1 kb DNA ladder
- Lane 2: Plasmid Vector (pSB1C3)
- Lane 3: Promoter and RBS (pVeg-SpoVG)
- Lane 4: spaIFEG Gene Cluster
- Lane 5: Double terminator
- Lane 6: 100 bp DNA ladder
Transformation of hyperspank and spoVG
Aim
To transform competent E.coli DH5α with hyperspank and spoVG.
Materials and Protocol
Please refer to transformation of E.coli.
Go back to our main Lab book page