Team:Newcastle/11 August 2010
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'''Figure #''': Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick. | '''Figure #''': Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick. | ||
- | * '''Lane 1''': | + | * '''Lane 1''': 1 kb DNA ladder |
* '''Lane 2''': Plasmid Vector (pSB1C3) | * '''Lane 2''': Plasmid Vector (pSB1C3) | ||
* '''Lane 3''': Promoter and RBS (pVeg-SpoVG) | * '''Lane 3''': Promoter and RBS (pVeg-SpoVG) | ||
* '''Lane 4''': ''spaIFEG'' Gene Cluster | * '''Lane 4''': ''spaIFEG'' Gene Cluster | ||
* '''Lane 5''': Double terminator | * '''Lane 5''': Double terminator | ||
- | * '''Lane 6''': | + | * '''Lane 6''': 100 bp DNA ladder |
=Transformation of hyperspank and spoVG= | =Transformation of hyperspank and spoVG= |
Revision as of 13:27, 11 August 2010
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Contents |
Gibson cloning of the rocF BioBrick
Aim
The aim of this experiment is to assemble the fragments we extracted yesterday in order to construct the rocF BioBrick and clone it into pSB1C3 in one step.
Subtilin Immunity BioBrick
Aims
Our aims for today are to run the gel electrophoresis to check whether we have the correct fragement sizes on the four parts that are we amplified yesterday. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts.
Materials and protocol
Please refer to the gel electrophoresis and the gel extraction protocols.
Results
Figure #: Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick.
- Lane 1: 1 kb DNA ladder
- Lane 2: Plasmid Vector (pSB1C3)
- Lane 3: Promoter and RBS (pVeg-SpoVG)
- Lane 4: spaIFEG Gene Cluster
- Lane 5: Double terminator
- Lane 6: 100 bp DNA ladder
Transformation of hyperspank and spoVG
Aim
To transform competent E.coli DH5α with hyperspank and spoVG.
Materials and Protocol
Please refer to transformation of E.coli.