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Team:Calgary/2 August 2010
From 2010.igem.org
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''Heritage Day: A Day off!!'' | ''Heritage Day: A Day off!!'' | ||
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| + | Set up a restriction digest of pRFP without PCR purification and left overnight. This was cut with EcoRI and SpeI. Since the sample had salts from PCR, concentration was not measured therefore a estimation of around 5.5uL to make the total concentration of the sample 10 uL. A PCR purification was made using older sample however even combining all the wells making the DNA volume in the tube 100uL, the concentrations were in their negatives. PCR purification will not be pursued further. Attempts will be made to plasmid switch PCR product without purification. | ||
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Revision as of 20:47, 3 August 2010
Notebook
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Monday August 2, 2010
Heritage Day: A Day off!!
Jeremy
Set up a restriction digest of pRFP without PCR purification and left overnight. This was cut with EcoRI and SpeI. Since the sample had salts from PCR, concentration was not measured therefore a estimation of around 5.5uL to make the total concentration of the sample 10 uL. A PCR purification was made using older sample however even combining all the wells making the DNA volume in the tube 100uL, the concentrations were in their negatives. PCR purification will not be pursued further. Attempts will be made to plasmid switch PCR product without purification.
No notebook page exists for this date. Sorry!
Retrieved from "http://2010.igem.org/Team:Calgary/2_August_2010"
