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Team:Newcastle/27 July 2010
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| - | + | ==Genomic DNA extraction experiment== | |
[[Image:Newcastle alan chromosome.jpg|thumb|200px|right]] | [[Image:Newcastle alan chromosome.jpg|thumb|200px|right]] | ||
[[Image:Newcastle ice chromosome.jpg|thumb|200px|right]] | [[Image:Newcastle ice chromosome.jpg|thumb|200px|right]] | ||
| - | + | ===Aims=== | |
The aim of today's experiment is to extract genomic DNA from both ''B. subtilis'' strains 168 and 3610. The genes necessary for the swarming biobrick and ''rocF'' biobrick will then hopefully be obtained from the genomic DNA using... | The aim of today's experiment is to extract genomic DNA from both ''B. subtilis'' strains 168 and 3610. The genes necessary for the swarming biobrick and ''rocF'' biobrick will then hopefully be obtained from the genomic DNA using... | ||
| - | + | ===Protocol=== | |
* Please refer to the [[Team:Newcastle/DNA extraction| DNA extraction of ''B. subtilis'']] page | * Please refer to the [[Team:Newcastle/DNA extraction| DNA extraction of ''B. subtilis'']] page | ||
| - | + | ===Discussion=== | |
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes. | At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes. | ||
| - | + | ===Conclusion=== | |
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010. | The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010. | ||
| - | + | ==Gel Electrophoresis== | |
[[Image:P7270470.JPG|thumb|300px|right]] | [[Image:P7270470.JPG|thumb|300px|right]] | ||
| - | + | ===Protocol=== | |
* Please refer to the [[Team:Newcastle/Gel electrophoresis|gel electrophoresis]] page for full protocol. | * Please refer to the [[Team:Newcastle/Gel electrophoresis|gel electrophoresis]] page for full protocol. | ||
| - | + | ===Discussion=== | |
* Unfortunately no bands were observed on the gel. | * Unfortunately no bands were observed on the gel. | ||
| - | + | ===Conclusion=== | |
As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long. | As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long. | ||
Revision as of 16:30, 30 July 2010
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Contents |
Genomic DNA extraction experiment
Aims
The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming biobrick and rocF biobrick will then hopefully be obtained from the genomic DNA using...
Protocol
- Please refer to the DNA extraction of B. subtilis page
Discussion
At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.
Conclusion
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
Gel Electrophoresis
Protocol
- Please refer to the gel electrophoresis page for full protocol.
Discussion
- Unfortunately no bands were observed on the gel.
Conclusion
As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.
   
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