Team:Tec-Monterrey/Headquarters

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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Ouruniversity">Our University</a></li>
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<h2>  Sponsors:</h2>
 
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<a href="http://www.uniparts.com.mx/"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a>
<a href="http://www.uniparts.com.mx/"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a>
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<h2>Follow us on:</h2>
 
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<h1>Project Summary</h1>
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<img src="https://static.igem.org/mediawiki/2010/2/23/Cb.jpg" alt="Centro de Biotecnología">
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Our headquarters for the development of this project were the facilities at the Center for Biotechnology FEMSA at Monterrey Institute of Technology and Higher Education (ITESM) in Monterrey, Mexico. At this center, we were kindly given access to the <b>Laboratory of Genetic Manipulation and Molecular Diagnostics</b>, which became our main workplace throughout the summer. It was in this laboratory that we had all the necessary equipment to perform most of our experiments: extractions, electrophoresis, ligations, digestions and purifications, as well as to carry out the necessary research for the development of the project.
 +
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      Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a>
+
We also had access to the <b>Fifth Floor Research Facilities at the Biotechnology Center</b>, where we performed the part of the project that demanded much more sophisticated equipment, such as transformations, quantifications and fluorescence assays.
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      The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration.
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      We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings.
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      With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.
+
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 +
Additionaly, part of our work had to be done at the <b>ITESM School of Medicine</b>, namely the preparation of electrocompetent cells.
 +
</p>
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<center>
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<p class="imgcenter">
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<h2> The Center for Biotechnology FEMSA</h2>
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<p class="blahimgder">
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<img src="https://static.igem.org/mediawiki/2010/8/89/Cbatras.jpg" alt="Centro de Biotecnología">
 +
This Center, located at Monterrey Institute of Technology and Higher Education (ITESM) in Monterrey, Mexico, is devoted to the generation of research, the transference of knowledge and the modelling of new business schemes that involve biotechnology and alimentary areas. For such reasons, it is equiped with cutting-edge infraestructure in laboratories, pilot plants and business incubators.
 +
</p>
 +
<p class="blahimgder">
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The Center emerged 15 years ago as a response to the increasing tendency in scientific and technologic innovation in the country and the whole world and through time it has supported the following lines of research, all focused in the innovation of products, processes and services:
 +
<ol>
 +
<li><b>Food Biotechnology:</b></li>
 +
<ul><li>Study of biomolecules found in Mexican plants and foods with therapeutic properties. </li>
 +
<li>Development and optimization of new food products and processes. </li>
 +
</ul>
 +
<li><b>Bioprocess Engineering:</b></li>
 +
<ul><li>Design, scalling and optimization of biotechnological processes. </li>
 +
</ul>
 +
<li><b>Pharmaceutical and Medical Biotechnology:</b> </li>
 +
<ul><li>Development and validation of new drugs. </li>
 +
<li>Molecular diagnostic of diseases. </li>
 +
<li>Culture and differentiation of tissues. </li>
 +
</ul>
 +
<li><b>Biological Chemistry:</b> </li>
 +
<ul><li>Isolation and characterization of biologically active compounds. </li>
 +
<li>Synthesis of new drugs</li>
 +
<li>Design of new materials with applications in the pharmaceutical and biotechnological industries. </li></ul>
 +
</ol>
 +
</p>
 +
<p class="blah">
 +
<b>Some of the strategic partners of the center are:</b>
 +
<ul>
 +
<li>Cambridge University, UK</li>
 +
<li>Texas A&M University</li>
 +
<li>Cornell University</li>
 +
<li>Arizona State University</li>
 +
<li>University of California (San Diego y Davis) </li>
 +
<li>Carnegie Mellon University</li>
 +
</ul>
 +
</p>
 +
<p class="blah">
 +
<b>Some of the achievements of the center are:</b>
 +
<ul>
 +
<li>More than 14 patent requests.</li>
 +
<li>More than 250 papers published in journals with high indexing level.</li>
 +
<li>3 published books.</li>
 +
<li>12 book chapters</li>
 +
<li>30 researches, of which, more than 70% belong to the National System of Researchers.
 +
</ul>
 +
</p>
 +
<center>
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<p class="imgcenter">
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<h2>References</h2>
 
-
<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690
 
-
<br><br>
 
-
<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522
 
-
<br>
 
-
<br>
 
-
<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012
 
-
<br><br>
 
-
<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258
 
-
<br><br></p>
 
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Revision as of 22:50, 17 October 2010

Tec de Monterrey





























Centro de Biotecnología Our headquarters for the development of this project were the facilities at the Center for Biotechnology FEMSA at Monterrey Institute of Technology and Higher Education (ITESM) in Monterrey, Mexico. At this center, we were kindly given access to the Laboratory of Genetic Manipulation and Molecular Diagnostics, which became our main workplace throughout the summer. It was in this laboratory that we had all the necessary equipment to perform most of our experiments: extractions, electrophoresis, ligations, digestions and purifications, as well as to carry out the necessary research for the development of the project.

We also had access to the Fifth Floor Research Facilities at the Biotechnology Center, where we performed the part of the project that demanded much more sophisticated equipment, such as transformations, quantifications and fluorescence assays.

Additionaly, part of our work had to be done at the ITESM School of Medicine, namely the preparation of electrocompetent cells.

The Center for Biotechnology FEMSA

Centro de Biotecnología This Center, located at Monterrey Institute of Technology and Higher Education (ITESM) in Monterrey, Mexico, is devoted to the generation of research, the transference of knowledge and the modelling of new business schemes that involve biotechnology and alimentary areas. For such reasons, it is equiped with cutting-edge infraestructure in laboratories, pilot plants and business incubators.

The Center emerged 15 years ago as a response to the increasing tendency in scientific and technologic innovation in the country and the whole world and through time it has supported the following lines of research, all focused in the innovation of products, processes and services:

  1. Food Biotechnology:
    • Study of biomolecules found in Mexican plants and foods with therapeutic properties.
    • Development and optimization of new food products and processes.
  2. Bioprocess Engineering:
    • Design, scalling and optimization of biotechnological processes.
  3. Pharmaceutical and Medical Biotechnology:
    • Development and validation of new drugs.
    • Molecular diagnostic of diseases.
    • Culture and differentiation of tissues.
  4. Biological Chemistry:
    • Isolation and characterization of biologically active compounds.
    • Synthesis of new drugs
    • Design of new materials with applications in the pharmaceutical and biotechnological industries.

Some of the strategic partners of the center are:

  • Cambridge University, UK
  • Texas A&M University
  • Cornell University
  • Arizona State University
  • University of California (San Diego y Davis)
  • Carnegie Mellon University

Some of the achievements of the center are:

  • More than 14 patent requests.
  • More than 250 papers published in journals with high indexing level.
  • 3 published books.
  • 12 book chapters
  • 30 researches, of which, more than 70% belong to the National System of Researchers.

Retrieved from "http://2010.igem.org/Team:Tec-Monterrey/Headquarters"