Team:Newcastle/27 July 2010

From 2010.igem.org

(Difference between revisions)
(Aims of genomic DNA extraction experiment)
(Materials)
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=====Materials=====
=====Materials=====
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# 50ml of 1% Agarose TAE
+
# 1% Agarose TAE
-
# 5µl SafeView
+
# SafeView
# TAE Buffer
# TAE Buffer
====Discussion====
====Discussion====
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 13:12, 27 July 2010

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Contents

Genomic DNA extraction experiment

Aim

The aim of today's experiment is to extract genomic DNA from Bacillus subtilis strain 3610 and 168,genes from which will be needed for the swarming biobrick and rocF biobrick.

Procedure

Discussion

At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.

Conclusion

Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.

Gel Electrophoresis

Protocol

Materials
  1. 1% Agarose TAE
  2. SafeView
  3. TAE Buffer

Discussion

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