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Team:Newcastle/26 August 2010
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Revision as of 01:12, 28 October 2010
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Screening Bacillius subtilis transformants
Aim
The aim of the experiment is to identify those colones that have the plasmid integrated at the correct position in the chromosome, which is the amyE locus. Those that have integrated at the correct position will not be able to break down starch, which can be tested by exposing the colonies on the starch plates to iodine.
Results
None of our colonies had halos, therefore the transformation and integration was successful.
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