Team:Heidelberg/Notebook/BSDesign
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See on our Notebook pages how we created binding site patterns. | See on our Notebook pages how we created binding site patterns. | ||
- | The adopted method for BS-patterns can be found on our [https://2010.igem.org/Team:Heidelberg/Notebook/Methods# | + | The adopted method for BS-patterns can be found on our [https://2010.igem.org/Team:Heidelberg/Notebook/Methods#random_assembly_PCR_.28raPCR.29 methods page]. |
On the [https://2010.igem.org/Team:Heidelberg/Parts#synthetic_microRNA_binding_Site_patterns_against_endogenous_miRNA Parts]-Page you can find standardized BS-patterns for hsa-mir-122 and has-mir-221, containing at least 2 binding sites. | On the [https://2010.igem.org/Team:Heidelberg/Parts#synthetic_microRNA_binding_Site_patterns_against_endogenous_miRNA Parts]-Page you can find standardized BS-patterns for hsa-mir-122 and has-mir-221, containing at least 2 binding sites. |
Revision as of 23:38, 27 October 2010
Binding Site DesignIntroductionTo create binding site (BS) patterns for micro RNAs (miRNAs) , we used the random assembly PCR (raPCR) – method from iGEM2009-Heidelberg team (see here) and adopted it to our purposes. The differences:
Several points need to be considered for setting up miRNA-binding site (miRBS) patterns:
The adopted method for BS-patterns can be found on our methods page. On the Parts-Page you can find standardized BS-patterns for hsa-mir-122 and has-mir-221, containing at least 2 binding sites.
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