Binding Site Design
To create binding site (BS) patterns for micro RNAs (miRNAs) , we used the random assembly PCR (raPCR) – method from iGEM2009-Heidelberg team (see here) and adopted it to our purposes. The differences:
Several points need to be considered for setting up miRNA-binding site (miRBS) patterns:
The adopted method for BS-patterns can be found on our methods page.
On the Parts-Page you can find standardized BS-patterns for hsa-mir-122 and has-mir-221, containing at least 2 binding sites.