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Team:METU Turkey/Characterization of an existing part
From 2010.igem.org
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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 591px; height: 313px;" alt="w7" src="https://static.igem.org/mediawiki/2010/3/3a/C1.GIF"></a></div> | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 591px; height: 313px;" alt="w7" src="https://static.igem.org/mediawiki/2010/3/3a/C1.GIF"></a></div> | ||
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| + | <br> The graph above shows the emission scan for RFP in part BBa_I13521. Since it was the best option in terms of fluorescence yield, 570 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 605 nm. | ||
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| + | <br> Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm. | ||
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| + | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 608px; height: 316px;" alt="w7" src="https://static.igem.org/mediawiki/2010/6/66/C2.GIF"></a></div> | ||
Revision as of 20:42, 27 October 2010
For parts BBa_I13521 and BBa_I13522, their excitation and emission maxima were determined. BBa_B0034 was used as a negative control. These data were used later in following characterization experiments as set parameters. The host was E. coli Top Ten in all experiments.
The graph above shows the emission scan for RFP in part BBa_I13521. Since it was the best option in terms of fluorescence yield, 570 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 605 nm.
Meanwhile, the graph below shows the emission scan for GFP in part BBa_I13522. Since it was the best option in terms of fluorescence yield, 395 nm was set as the excitation wavelength. As it can be seen from the graph, maximum emission wavelength is 515 nm.
