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Team:METU Turkey/Planning
From 2010.igem.org
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| + | <br> | ||
| + | <br>PROJECT EXECUTION | ||
| + | <br> | ||
| + | |||
| + | <br>Design and Synthesis of Response Elements | ||
| + | <br> | ||
| + | <br> - Construction of Cell Sensor modules | ||
| + | <br> - Preparation of competent cell | ||
| + | <br> - Design and ordering of DNA parts | ||
| + | <br> - Cloning | ||
| + | <br> - Validation | ||
| + | <br> - Assembly of final construct | ||
| + | |||
| + | <br> | ||
| + | <br>Cell Sensor Experiments | ||
| + | <br> - Fermentor studies | ||
| + | <br> - Flask experiments | ||
| + | <br> - Solid culture experiments | ||
| + | <br> - Myoglobin assay optimization | ||
| + | <br> - Signal quantification with fluorescence spectroscopy | ||
| + | <br> - Imaging with confocal laser scanning microscopy | ||
| + | |||
| + | <br> | ||
| + | <br>CooA Expression and Purification | ||
| + | <br> - Overexpression optimization | ||
| + | <br> - Purification optimization | ||
| + | |||
| + | <br> | ||
| + | <br>Characterization: Isothermal Titration Calorimetry (ITC) | ||
| + | <br> - DNA component preparation | ||
| + | <br> - Optimization studies | ||
| + | <br> - Determination of Response Element - Transcription Factor Affinities | ||
| + | |||
| + | <br> | ||
| + | <br>Characterization: Electrophoretic Mobility Shift Assay (EMSA) | ||
| + | <br> - Optimization studies | ||
| + | <br> - Determination of Response Element - Transcription Factor Affinities | ||
| + | |||
| + | <br> | ||
| + | <br>Characterization: Intrinsic Tryptophan Fluorescence (ITF) | ||
| + | <br> - Optimization studies | ||
| + | <br> - Determination of Response Element - Transcription Factor Affinities | ||
| + | <br> | ||
Revision as of 20:19, 27 October 2010
PROJECT EXECUTION
Design and Synthesis of Response Elements
- Construction of Cell Sensor modules
- Preparation of competent cell
- Design and ordering of DNA parts
- Cloning
- Validation
- Assembly of final construct
Cell Sensor Experiments
- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy
CooA Expression and Purification
- Overexpression optimization
- Purification optimization
Characterization: Isothermal Titration Calorimetry (ITC)
- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Electrophoretic Mobility Shift Assay (EMSA)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
Characterization: Intrinsic Tryptophan Fluorescence (ITF)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities
