"
Team:Chiba/System 1/Testing components
From 2010.igem.org
(Difference between revisions)
| Line 350: | Line 350: | ||
----------------------------------------------------------------------------------- --> | ----------------------------------------------------------------------------------- --> | ||
<br><br><br> | <br><br><br> | ||
| + | ===Explanation of Circuit=== | ||
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input. | To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input. | ||
The subparts of those plasmids are shown in Fig.1. | The subparts of those plasmids are shown in Fig.1. | ||
Revision as of 19:44, 27 October 2010
===Explanation of Circuit=== To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The subparts of those plasmids are shown in Fig.1.
