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Team:Tsinghua/Notebook/9 October 2010
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== Module I, Group 1A == | == Module I, Group 1A == | ||
Colony pcr to screen the clones in which landing pad has successfully integrated into genome, using up primers from the plasmid and down primers from the genome. | Colony pcr to screen the clones in which landing pad has successfully integrated into genome, using up primers from the plasmid and down primers from the genome. | ||
| + | Select the positive clones to amplify in LB media with 0.03% tetracyclin. | ||
| + | |||
== Module I, Group 2c == | == Module I, Group 2c == | ||
After purification, ligation reaction took place in a mixed system as following， respectively. | After purification, ligation reaction took place in a mixed system as following， respectively. | ||
Latest revision as of 15:35, 27 October 2010
Module I, Group 1A
Colony pcr to screen the clones in which landing pad has successfully integrated into genome, using up primers from the plasmid and down primers from the genome. Select the positive clones to amplify in LB media with 0.03% tetracyclin.
Module I, Group 2c
After purification, ligation reaction took place in a mixed system as following， respectively.
eGFP 3ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 12ul
mCherry 2ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 13ul
Kan 3ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 12ul
Incubate at 16 centidegree overnight.
