Team:Alberta/Tour/biobytes

From 2010.igem.org

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We continued to develop the BioBytes assembly method developed by last year’s team.
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<p>
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The method has three main components:
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This year's project needed a new approach to assembling DNA constructs. This need for a fast and efficient assembly standard was filled by BioByte 2.0. As such Biobyte 2.0 represents an innovative step forward from last year's BioByte 1.0.
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It utilized iron micro beads to anchor the DNA which allows for the DNA pieces to be added in a controlled sequential manner ensuring the proper orientation and order of parts. This is made possible because of the design of our unique anchor part. When paired with our unique terminal cap part will allow the assembly to be circularized and transformed into ''E. coli''
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==The Anchor==
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An iron micro bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct.  The bead allows us to manipulate the DNA with magnets, making washing and subsequent attachments easier.
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[[Image:team-alberta-anchor-tour.jpg|center]]
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==The Bytes==
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DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA.  The A end can join only with another A end and the B end can join only with another B end.  As a result, pieces can only be joined in a single orientation.
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[[Image:team-alberta-bytes-tour.jpg|center]]
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==The cap==
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A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
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[[Image:team-alberta-cap-tour.jpg|center]]
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==Putting the pieces together==
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The process of building a plasmid is more elegant and more rapid than the current BioByte system! 
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[[Image:team-alberta-biobyteprocess-tour.jpg]]
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Easy! And fast too, each step takes about 7 minutes.
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[[Image:team-alberta-building-tour.jpg|center]]
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Compare that to the standard BioBrick method:
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[[Image:team-alberta-biobrick-tour.jpg|center]]
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Using this process we were able to assemble eight pieces in an afternoon!
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Revision as of 01:29, 27 October 2010

TEAM ALBERTA

This year's project needed a new approach to assembling DNA constructs. This need for a fast and efficient assembly standard was filled by BioByte 2.0. As such Biobyte 2.0 represents an innovative step forward from last year's BioByte 1.0.

It utilized iron micro beads to anchor the DNA which allows for the DNA pieces to be added in a controlled sequential manner ensuring the proper orientation and order of parts. This is made possible because of the design of our unique anchor part. When paired with our unique terminal cap part will allow the assembly to be circularized and transformed into E. coli


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