UTDallas/25 October 2010

From 2010.igem.org

(Difference between revisions)
(→October 25, 2010)
(→October 25, 2010)
 
Line 47: Line 47:
*Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene
*Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene
*Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow
*Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow
 +
;Alkane Sensor
 +
*Set up another digestion of the midipreped plasmis of part E0240 with XbaI.
 +
*65C 10mins to heat kill XbaI
 +
*Nucleotide removal kit after that to cleanup the DNA
 +
*Tried blunt End ligation. So did blunt ending with Klenow fragment of the Vector(E0240) and the insert (PAlk Promoter)
 +
*Nucleotide removal kit after that to cleanup the DNA of both vector ans the insert
 +
*Setup ligation at room temperature for 1 hour.
 +
*Transformed into Dh5Alpha cells and plated them in 50ug/ml agar plate.

Latest revision as of 20:48, 27 October 2010


Notebook

June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=UTDallas/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=UTDallas/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=UTDallas/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=UTDallas/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=UTDallas/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=UTDallas/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=UTDallas/28_June_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_June_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_June_2010&action=edit 30]
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=UTDallas/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=UTDallas/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=UTDallas/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_July_2010&action=edit 16] [http://2010.igem.org/UTDallas/17_July_2010 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=UTDallas/19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_July_2010&action=edit 25]
[http://2010.igem.org/UTDallas/26_July_2010 26] [http://2010.igem.org/UTDallas/27_July_2010 27] [http://2010.igem.org/UTDallas/28_July_2010 28] [http://2010.igem.org/UTDallas/29_July_2010 29] [http://2010.igem.org/UTDallas/30_July_2010 30] [http://2010.igem.org/wiki/index.php?title=UTDallas/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=UTDallas/1_August_2010&action=edit 1]
[http://2010.igem.org/UTDallas/2_August_2010 2] [http://2010.igem.org/UTDallas/3_August_2010 3] [http://2010.igem.org/UTDallas/4_August_2010 4] [http://2010.igem.org/UTDallas/5_August_2010 5] [http://2010.igem.org/UTDallas/6_August_2010 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=UTDallas/8_August_2010&action=edit 8]
[http://2010.igem.org/UTDallas/9_August_2010 9] [http://2010.igem.org/UTDallas/10_August_2010 10] [http://2010.igem.org/UTDallas/11_August_2010 11] [http://2010.igem.org/UTDallas/12_August_2010 12] [http://2010.igem.org/UTDallas/13_August_2010 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=UTDallas/15_August_2010&action=edit 15]
[http://2010.igem.org/UTDallas/16_August_2010 16] [http://2010.igem.org/UTDallas/17_August_2010 17] [http://2010.igem.org/UTDallas/18_August_2010 18] [http://2010.igem.org/UTDallas/19_August_2010 19] [http://2010.igem.org/UTDallas/20_August_2010 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=UTDallas/22_August_2010&action=edit 22]
[http://2010.igem.org/UTDallas/23_August_2010 23] [http://2010.igem.org/UTDallas/24_August_2010 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_August_2010&action=edit 25] [http://2010.igem.org/UTDallas/26_August_2010 26] [http://2010.igem.org/UTDallas/27_August_2010 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_August_2010&action=edit 29]
[http://2010.igem.org/UTDallas/30_August_2010 30] [http://2010.igem.org/UTDallas/31_August_2010 31]
September
MTWTFSS
    [http://2010.igem.org/UTDallas/1_September_2010 1] [http://2010.igem.org/UTDallas/2_September_2010 2] [http://2010.igem.org/UTDallas/3_September_2010 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=UTDallas/6_September_2010&action=edit 6] [http://2010.igem.org/UTDallas/7_September_2010 7] [http://2010.igem.org/UTDallas/8_September_2010 8] [http://2010.igem.org/UTDallas/9_September_2010 9] [http://2010.igem.org/UTDallas/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_September_2010&action=edit 12]
[http://2010.igem.org/UTDallas/13_September_2010 13] [http://2010.igem.org/UTDallas/14_September_2010 14] [http://2010.igem.org/UTDallas/15_September_2010 15] [http://2010.igem.org/UTDallas/16_September_2010 16] [http://2010.igem.org/UTDallas/17_September_2010 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_September_2010&action=edit 19]
[http://2010.igem.org/UTDallas/20_September_2010 20] [http://2010.igem.org/UTDallas/21_September_2010 21] [http://2010.igem.org/UTDallas/22_September_2010 22] [http://2010.igem.org/UTDallas/23_September_2010 23] [http://2010.igem.org/UTDallas/24_September_2010 24] [http://2010.igem.org/UTDallas/25_September_2010 25] [http://2010.igem.org/UTDallas/26_September_2010 26]
[http://2010.igem.org/UTDallas/27_September_2010 27] [http://2010.igem.org/UTDallas/28_September_2010 28] [http://2010.igem.org/UTDallas/29_September_2010 29] [http://2010.igem.org/UTDallas/30_September_2010 30]
October
MTWTFSS
        [http://2010.igem.org/UTDallas/1_October_2010 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=UTDallas/4_October_2010&action=edit 4] [http://2010.igem.org/UTDallas/5_October_2010 5] [http://2010.igem.org/UTDallas/6_October_2010 6] [http://2010.igem.org/UTDallas/7_October_2010 7] [http://2010.igem.org/UTDallas/8_October_2010 8] [http://2010.igem.org/UTDallas/9_October_2010 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_October_2010&action=edit 10]
[http://2010.igem.org/UTDallas/11_October_2010 11] [http://2010.igem.org/UTDallas/12_October_2010 12] [http://2010.igem.org/UTDallas/13_October_2010 13] [http://2010.igem.org/UTDallas/14_October_2010 14] [http://2010.igem.org/UTDallas/15_October_2010 15] [http://2010.igem.org/UTDallas/16_October_2010 16] [http://2010.igem.org/UTDallas/17_October_2010 17]
[http://2010.igem.org/UTDallas/18_October_2010 18] [http://2010.igem.org/UTDallas/19_October_2010 19] [http://2010.igem.org/UTDallas/20_October_2010 20] [http://2010.igem.org/UTDallas/21_October_2010 21] [http://2010.igem.org/UTDallas/22_October_2010 22] [http://2010.igem.org/UTDallas/23_October_2010 23] [http://2010.igem.org/UTDallas/24_October_2010 24]
[http://2010.igem.org/UTDallas/25_October_2010 25] [http://2010.igem.org/UTDallas/26_October_2010 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=UTDallas/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=UTDallas/31_October_2010&action=edit 31]
November
MTWTFSS
[http://2010.igem.org/wiki/index.php?title=UTDallas/1_November_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=UTDallas/2_November_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=UTDallas/3_November_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=UTDallas/4_November_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=UTDallas/5_November_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=UTDallas/6_November_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=UTDallas/7_November_2010&action=edit 7]
[http://2010.igem.org/wiki/index.php?title=UTDallas/8_November_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=UTDallas/9_November_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=UTDallas/10_November_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=UTDallas/11_November_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=UTDallas/12_November_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=UTDallas/13_November_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=UTDallas/14_November_2010&action=edit 14]
[http://2010.igem.org/wiki/index.php?title=UTDallas/15_November_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=UTDallas/16_November_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=UTDallas/17_November_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=UTDallas/18_November_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=UTDallas/19_November_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=UTDallas/20_November_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=UTDallas/21_November_2010&action=edit 21]
[http://2010.igem.org/wiki/index.php?title=UTDallas/22_November_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=UTDallas/23_November_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=UTDallas/24_November_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=UTDallas/25_November_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=UTDallas/26_November_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=UTDallas/27_November_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=UTDallas/28_November_2010&action=edit 28]
[http://2010.igem.org/wiki/index.php?title=UTDallas/29_November_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=UTDallas/30_November_2010&action=edit 30]


October 25, 2010

  • Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene
  • Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow
Alkane Sensor
  • Set up another digestion of the midipreped plasmis of part E0240 with XbaI.
  • 65C 10mins to heat kill XbaI
  • Nucleotide removal kit after that to cleanup the DNA
  • Tried blunt End ligation. So did blunt ending with Klenow fragment of the Vector(E0240) and the insert (PAlk Promoter)
  • Nucleotide removal kit after that to cleanup the DNA of both vector ans the insert
  • Setup ligation at room temperature for 1 hour.
  • Transformed into Dh5Alpha cells and plated them in 50ug/ml agar plate.
Retrieved from "http://2010.igem.org/UTDallas/25_October_2010"