UTDallas/25 October 2010

From 2010.igem.org


Notebook

June
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


October 25, 2010

  • Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene
  • Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow
Alkane Sensor
  • Set up another digestion of the midipreped plasmis of part E0240 with XbaI.
  • 65C 10mins to heat kill XbaI
  • Nucleotide removal kit after that to cleanup the DNA
  • Tried blunt End ligation. So did blunt ending with Klenow fragment of the Vector(E0240) and the insert (PAlk Promoter)
  • Nucleotide removal kit after that to cleanup the DNA of both vector ans the insert
  • Setup ligation at room temperature for 1 hour.
  • Transformed into Dh5Alpha cells and plated them in 50ug/ml agar plate.