Team:Tokyo Metropolitan/Project/Fiber/Protocol
From 2010.igem.org
(Difference between revisions)
(→Protocol3:PCR) |
(→Protocol3:PCR) |
||
Line 99: | Line 99: | ||
<li>Taq polymerase<br/> | <li>Taq polymerase<br/> | ||
</ul> | </ul> | ||
- | <br> | + | <br/> |
+ | |||
<font size="3"><span style="text-decoration:underline">Equipment</font></span> | <font size="3"><span style="text-decoration:underline">Equipment</font></span> | ||
<ul> | <ul> | ||
Line 108: | Line 109: | ||
<li>pipette tip | <li>pipette tip | ||
</ul> | </ul> | ||
- | <br> | + | <br/> |
+ | |||
<font size="3"><span style="text-decoration:underline">Procedure</font></span><br> | <font size="3"><span style="text-decoration:underline">Procedure</font></span><br> | ||
<ol><li>Add and mix above materials to each PCR tubes on the ice</li> | <ol><li>Add and mix above materials to each PCR tubes on the ice</li> |
Revision as of 19:30, 26 October 2010
E.coli Fiber Project Protocol
Protocol1:Grow up a culture of A.xylinum
Material
Equipment
Procedure
Note
|
Protocol2:Grow up a culture of E.coli
Material
Equipment
Procedure
|
Protocol3:PCR
Material
|
Protocol4:Agarose gel electrophoresis
Material
Equipment
Procedure
|
Protocol5-1:DNA purification from agarose gel
Material
Equipment
Procedure
|
Protocol5-2:DNA Purification with silica gel
Material
- Binding buffer
- silica gel
- wash buffer
- TE buffer
Equipment
- centrifuge
- vortex
- aspirator
- pipette
- pipette tip
Procedure
- Add 3 times Binding buffer than digestion production
- Add 10µl of silica gel and mix with Vortex
- Centrifuge 1min
- Remove supernatant with aspirator
- Add Wash buffer and mix with vortex
- Centrifuge 30sec
- Remove supernatant with aspirator
- Remove ethanol by drying
- Add TE buffer and mix with Vortex
- Centrifuge 30sec
- DNA is solved in Supernatant
Protocol6:Restriction enzyme digestion
Material
Equipment
Procedure
|
Protocol7:Ligation
Material
Equipment
Procedure
|
Protocol8:Transformation
Material
Equipment
Procedure
|
Protocol9:Miniprep (extraction of plasmid kit)
Material
Equipment
Procedure
|