Team:Alberta/Tour/biobytes

From 2010.igem.org

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The method has three main components:
The method has three main components:
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[[Image:team-alberta-anchor-tour.jpg|float=left]]
 
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<div class="tour-info">
 
==The Anchor==
==The Anchor==
A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct.  The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct.  The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.
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</div>
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[[Image:team-alberta-anchor-tour.jpg|center]]  
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<div class="tour-info">
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[[Image:team-alberta-bytes-tour.jpg|float=left]]  
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==The bytes==  
==The bytes==  
DNA fragments that can be attached together to build up a larger construct. There are two types  of pieces, AB and BA.  The A end can join only with another A end and the B end can join only with another B end.  As a result pieces can only be joined in a single orientation.  
DNA fragments that can be attached together to build up a larger construct. There are two types  of pieces, AB and BA.  The A end can join only with another A end and the B end can join only with another B end.  As a result pieces can only be joined in a single orientation.  
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</div>
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[[Image:team-alberta-bytes-tour.jpg|center]]  
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<div class="tour-info">
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[[Image:team-alberta-cap-tour.jpg|float=left]]  
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==The cap==
==The cap==
A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.
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</div>
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[[Image:team-alberta-cap-tour.jpg|center]]  
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<div class="tour-info">
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[[Image:team-alberta-biobrick-tour.jpg]]
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==Putting the pieces together==
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[[Image:team-alberta-building-tour.jpg]]
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The process of building a plasmid is more elegant and more rapid than the current biobyte system!   
The process of building a plasmid is more elegant and more rapid than the current biobyte system!   
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</div>
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<div class="tour-info">
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[[Image:team-alberta-biobyteprocess-tour.jpg]]  
[[Image:team-alberta-biobyteprocess-tour.jpg]]  
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Starting with an anchor, add the first piece and ligate.
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Easy! And fast too, each step takes about 7 minutes.  
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Then hold the single piece construct in the tube by placing it on the magnetic rack.  Now you can wash away most of the excess of piece 1.
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[[Image:team-alberta-building-tour.jpg|center]]
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Add the next piece and repeat until you have added all the pieces you want.
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Compare that to the standard biobrick method:
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Then add the cap.
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[[Image:team-alberta-biobrick-tour.jpg|center]]
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Now just heat to release the anchor and open up the cap, upon cooling the construct will circularize. 
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Using this process we were able to assemble eight pieces in an afternoon!
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Easy!
 
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Using this process we were able to assemble eight pieces in an afternoon!
 
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</div>
 
{{Team:Alberta/endMainContent|class=not-top}}
{{Team:Alberta/endMainContent|class=not-top}}

Revision as of 15:42, 26 October 2010

TEAM ALBERTA

We continued to develop the biobytes assembly method developed by last year’s team. The method has three main components:

The Anchor

A ferro-magnetic bead attached to a piece of DNA. This piece serves as the initial piece from which we assemble a DNA construct. The bead allows us to manipulate the DNA with magnets making washing and subsequent attachments easier.

Team-alberta-anchor-tour.jpg

The bytes

DNA fragments that can be attached together to build up a larger construct. There are two types of pieces, AB and BA. The A end can join only with another A end and the B end can join only with another B end. As a result pieces can only be joined in a single orientation.

Team-alberta-bytes-tour.jpg

The cap

A DNA fragment that finishes off a construct and allows for circularization of the construct into a plasmid.

Team-alberta-cap-tour.jpg

Putting the pieces together

The process of building a plasmid is more elegant and more rapid than the current biobyte system!

Team-alberta-biobyteprocess-tour.jpg

Easy! And fast too, each step takes about 7 minutes.

Team-alberta-building-tour.jpg

Compare that to the standard biobrick method:

Team-alberta-biobrick-tour.jpg

Using this process we were able to assemble eight pieces in an afternoon!