Team:SDU-Denmark/project-p

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(Motility assay)
(Motility assay)
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The duplicate of the motility assay shows: <br>
The duplicate of the motility assay shows: <br>
* very little motility of the non-flagellated negative control DH5alpha. Less than in the first assay <br>
* very little motility of the non-flagellated negative control DH5alpha. Less than in the first assay <br>
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* Minimal motility of the wild type MG1655, though they have colonized a bigger area on the plate than DH5aplha. The MG1655 cells also show less motility in this assay than in the first. Also the colony morphorlogy is wvery different in the two assays<br> The only difference between the two assays is that the second assay was plated out and grown on plates in a class II lab because of the positive control. <br>
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* Minimal motility of the wild type MG1655, though they have colonized a bigger area on the plate than DH5aplha. The MG1655 cells also show less motility in this assay than in the first. Also the colony morphorlogy is very different in the two assays<br> The only difference between the two assays is that the second assay was plated out and grown on plates in a class II lab because of the positive control, H10407, is classified as a class two bacteria. <br>
* Greate motility of the hyper flagellated ''E. coli'' strain H10407 (class II pathogen) positive control <br>
* Greate motility of the hyper flagellated ''E. coli'' strain H10407 (class II pathogen) positive control <br>
* MG1655 cells with pSB1C3-K343004 have moved farther than both the negative control and the wild type, but not as far as the positive control. On both assays of this transformant a "line" is visable arround the edge of the colony, we think this is an indication that these cells are not moving much further. This transformant show similar motility as in the first assay<br>
* MG1655 cells with pSB1C3-K343004 have moved farther than both the negative control and the wild type, but not as far as the positive control. On both assays of this transformant a "line" is visable arround the edge of the colony, we think this is an indication that these cells are not moving much further. This transformant show similar motility as in the first assay<br>

Revision as of 22:07, 25 October 2010