Formal Meeting - 21st October

• Apologies - Phil, Younus, Deena (late)

yneA Characteristaion:

• ΔamyE:Pspac(hy)::yneA

• B. subtilis spread of IPTG may not be homogenous through the population.
• E. coli has transporter deltions: check E. coli wiki

• take out pMap65 results

• Median, Mode add to graph

• pMutin4 proportion of cells

• 20% responding- looks like

• Biomass of cells - length * amount (counts)) against IPTG

• Lysis acts to counter the overexpression of yneA ... so we have fewer cells

• Younus AP: send script for yneA to Jen, Matt, Neil

• Plot: Max cell length against ITPG + Error bars

Arabinose

• We think we have it cloned infront of GFP
• B. subtilis transformation tommorrow
• Fluorescence
• Characterisation of previous part!!!

Concrete

• Damaged building - earthquake

Flux balance

• difference between optimal and normal

Lab work

• DNA submitted - tracking number sent
• 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

• Deena and Harsh

• Risk assessment - Bacillus

• BioCOSH

• Safety Guy talk mention it

Presentation

• Phil - DON'T BE AGGRESSIVE!!!!
• Beginning:
  • say what the project is about
  • mention Bacillus subtilis
  • Why bacillus?
  • bioconcrete- show its current
  • Non-pathogenic not non-virulent
• BioBricks:
  • Previous work on CaCO₃
  • Don't use the word PROVES
  • Alan - Flux first, speak slowly
• Gold:
  • Jannetta write RFC
  • number on judging form
  • webservice!!!
  • Separate slid for work flows
• SEM:
  • Images are ours
• Summary table
  • redo with BRONZE, SILVER, GOLD section?!

Other

• Insurance form
• Taxi
• Younus - Poster next thursday due in?
• Action points:
  • Check presentation and poster submission!!!
  • Phil and Rachel add characteristation data to the wiki