Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
(Difference between revisions)
Line 29: | Line 29: | ||
==Arabinose== | ==Arabinose== | ||
- | We think we have it cloned infront of GFP | + | * We think we have it cloned infront of GFP |
- | '' | + | *''B. subtilis'' transformation tommorrow |
- | Fluorescence | + | *Fluorescence |
- | '''Characterisation of previous part!!!''' | + | *'''Characterisation of previous part!!!''' |
==Concrete== | ==Concrete== | ||
Line 44: | Line 44: | ||
==Lab work== | ==Lab work== | ||
- | DNA submitted... tracking number sent | + | *DNA submitted... tracking number sent |
- | 1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! | + | *1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted! |
==Safety page== | ==Safety page== | ||
- | Deena and Harsh | + | *Deena and Harsh |
- | Risk assessment- ''Bacillus'' | + | *Risk assessment - ''Bacillus'' |
- | BioCOSH | + | *BioCOSH |
- | Safety Guy talk mention it | + | *Safety Guy talk mention it |
==Presentation== | ==Presentation== | ||
- | + | *Phil - DON'T BE AGGRESSIVE!!!! | |
- | Beginning: | + | *Beginning: |
- | *say what the project is about | + | **say what the project is about |
- | *mention ''Bacillus subtilis'' | + | **mention ''Bacillus subtilis'' |
- | *Why bacillus? | + | **Why bacillus? |
- | *bioconcrete- show its current | + | **bioconcrete- show its current |
- | *Non-pathogenic not non-virulent | + | **Non-pathogenic not non-virulent |
- | *Previous work on CaCO<sub>3</sub> | + | **Previous work on CaCO<sub>3</sub> |
- | *Don't use the word '''PROVES''' | + | **Don't use the word '''PROVES''' |
- | *Separate slid for work flows | + | **Separate slid for work flows |
- | *Alan - Flux first, speak slowly | + | **Alan - Flux first, speak slowly |
- | *Summary table- redo | + | **Summary table- redo |
- | *BRONZE, SILVER, GOLD!!!!!!! | + | **BRONZE, SILVER, GOLD!!!!!!! |
- | + | *Gold | |
- | + | **Jannetta write RFC | |
- | Jannetta write RFC | + | **number on judging form |
- | *number on judging form | + | **webservice!!! |
- | *webservice!!! | + | * |
- | + | **Images are ours | |
- | + | ||
- | Images are ours | + | |
==Other== | ==Other== | ||
- | Insurance form | + | *Insurance form |
- | Taxi | + | *Taxi |
- | + | *Younus - Poster next thursday due in? | |
- | Younus - Poster next thursday due in? | + | *Action point check presentation and poster submission!!! |
- | + | ||
- | Action point check presentation and poster submission!!! | + | |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 16:25, 25 October 2010
|
- Apologies - Phil, Younus, Deena late
- Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
- We think we have it cloned infront of GFP
- B. subtilis transformation tommorrow
- Fluorescence
- Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
- DNA submitted... tracking number sent
- 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
- Deena and Harsh
- Risk assessment - Bacillus
- BioCOSH
- Safety Guy talk mention it
Presentation
- Phil - DON'T BE AGGRESSIVE!!!!
- Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- Previous work on CaCO3
- Don't use the word PROVES
- Separate slid for work flows
- Alan - Flux first, speak slowly
- Summary table- redo
- BRONZE, SILVER, GOLD!!!!!!!
- Gold
- Jannetta write RFC
- number on judging form
- webservice!!!
-
- Images are ours
Other
- Insurance form
- Taxi
- Younus - Poster next thursday due in?
- Action point check presentation and poster submission!!!