Team:Newcastle/Meetings/21 October 2010

From 2010.igem.org

(Difference between revisions)
Line 29: Line 29:
==Arabinose==  
==Arabinose==  
-
We think we have it cloned infront of GFP  
+
* We think we have it cloned infront of GFP  
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''Bacillus subtilis'' transformation tommorrow  
+
*''B. subtilis'' transformation tommorrow  
-
Fluorescence  
+
*Fluorescence  
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'''Characterisation of previous part!!!'''  
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*'''Characterisation of previous part!!!'''  
==Concrete==  
==Concrete==  
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==Lab work==  
==Lab work==  
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DNA submitted... tracking number sent  
+
*DNA submitted... tracking number sent  
-
1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted!
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*1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted!
==Safety page==  
==Safety page==  
-
Deena and Harsh  
+
*Deena and Harsh  
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Risk assessment- ''Bacillus''  
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*Risk assessment - ''Bacillus''  
-
BioCOSH  
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*BioCOSH  
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Safety Guy talk mention it  
+
*Safety Guy talk mention it  
==Presentation==
==Presentation==
-
'''Phil''' DON'T BE AGGRESSIVE!!!!
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*Phil - DON'T BE AGGRESSIVE!!!!
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Beginning:  
+
*Beginning:  
-
*say what the project is about  
+
**say what the project is about  
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*mention ''Bacillus subtilis''
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**mention ''Bacillus subtilis''
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*Why bacillus?  
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**Why bacillus?  
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*bioconcrete- show its current
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**bioconcrete- show its current
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*Non-pathogenic not non-virulent  
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**Non-pathogenic not non-virulent  
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*Previous work on CaCO<sub>3</sub>
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**Previous work on CaCO<sub>3</sub>
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*Don't use the word '''PROVES'''  
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**Don't use the word '''PROVES'''  
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*Separate slid for work flows  
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**Separate slid for work flows  
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*Alan - Flux first, speak slowly
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**Alan - Flux first, speak slowly
-
*Summary table- redo
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**Summary table- redo
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*BRONZE, SILVER, GOLD!!!!!!! (see below)
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**BRONZE, SILVER, GOLD!!!!!!!
-
 
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*Gold
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===GOLD===
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**Jannetta write RFC
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Jannetta write RFC
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**number on judging form  
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*number on judging form  
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**webservice!!!
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*webservice!!!
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*
-
 
+
**Images are ours
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===SEM===
+
-
Images are ours
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==Other==
==Other==
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Insurance form
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*Insurance form
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Taxi  
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*Taxi  
-
 
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*Younus - Poster next thursday due in?   
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Younus - Poster next thursday due in?   
+
*Action point check presentation and poster submission!!!
-
 
+
-
Action point check presentation and poster submission!!!
+
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 16:25, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map
  • Apologies - Phil, Younus, Deena late
  • Action point: Phil and Rachel add characteristation data to the wiki

Contents

yneA Characteristaion:

  • ΔamyE:Pspac(hy)::yneA
  • B. subtilis spread of IPTG may not be homogenous through the population.
  • E. coli has transporter deltions: check E. coli wiki
  • take out pMap65 results
  • Median, Mode add to graph
  • pMutin4 proportion of cells
  • 20% responding- looks like
  • Biomass of cells - length * amount (counts)) against IPTG
  • Lysis acts to counter the overexpression of yneA ... so we have fewer cells
  • Younus AP: send script for yneA to Jen, Matt, Neil
  • Plot: Max cell length against ITPG + Error bars

Arabinose

  • We think we have it cloned infront of GFP
  • B. subtilis transformation tommorrow
  • Fluorescence
  • Characterisation of previous part!!!

Concrete

  • Damaged building - earthquake

Flux balance

  • difference between optimal and normal

Lab work

  • DNA submitted... tracking number sent
  • 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

  • Deena and Harsh
  • Risk assessment - Bacillus
  • BioCOSH
  • Safety Guy talk mention it

Presentation

  • Phil - DON'T BE AGGRESSIVE!!!!
  • Beginning:
    • say what the project is about
    • mention Bacillus subtilis
    • Why bacillus?
    • bioconcrete- show its current
    • Non-pathogenic not non-virulent
    • Previous work on CaCO3
    • Don't use the word PROVES
    • Separate slid for work flows
    • Alan - Flux first, speak slowly
    • Summary table- redo
    • BRONZE, SILVER, GOLD!!!!!!!
  • Gold
    • Jannetta write RFC
    • number on judging form
    • webservice!!!
    • Images are ours

Other

  • Insurance form
  • Taxi
  • Younus - Poster next thursday due in?
  • Action point check presentation and poster submission!!!
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