Team:Newcastle/Meetings/21 October 2010

Apologies - Phil, Younus, Deena late

Action point: Phil and Rachel add characteristation data to the wiki

yneA Characteristaion:

• ΔamyE:Pspac(hy)::yneA

• Bacillus subtilis spread of IPTG may not be homogenous through the population.
• E.coli has transporter deltions: check E.coli wiki

take out pMap65 results

Median, Mode add to graph

pMutin4 proportion of cells

20% responding- looks like

Biomass of cells - length * amount (counts)) against IPTG

Lysis acts to counter the overexpression of yneA ... so we have fewer cells

Younus AP: send script for yneA to Jen, Matt, Neil

Plot: Max cell length against ITPG + Error bars

Arabinose

We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!

Concrete

Damaged building- earthquake

Flux balance

difference between optimal and normal

Lab work

DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

Deena and Harsh

Risk assessment- Bacillus

BioCOSH

Safety Guy talk mention it

Presentation

Phil DON'T BE AGGRESSIVE!!!!

Beginning:

• say what the project is about
• mention Bacillus subtilis
• Why bacillus?
• bioconcrete- show its current

Non-pathogenic not non-virulent Previous work on CaCO3 Don't use the word PROVES Separate slid for work flows

Alan - Flux first, speak slowly

Summary table- redo

BRONZE, SILVER, GOLD!!!!!!!

GOLD

Jannetta write RFC

• number on judging form
• webservice!!!

SEM

Images are ours

Other

Insurance form Taxi

Younus - Poster next thursday due in?

Action point check presentation and poster submission!!!