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Team:Newcastle/5 July 2010
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==Aims== | ==Aims== | ||
| - | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein ( | + | *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (''rfp''). |
==Materials== | ==Materials== | ||
Revision as of 15:02, 25 October 2010
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Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (rfp).
Materials
- Refrigerated transformation plates.
Protocol
- Set up a overnight culture of colonies transformed with pMutin4 and pSB1AT3.
Inference
- Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
   
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