Team:Caltech/Week 3

(Difference between revisions)

Revision as of 01:24, 29 June 2010

iGEM 2010

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support

Tested the success of the construct we made using digest and gel electrophoresis.
- Digested ligation product from 6/26 with EcoRI and PstI.
- Ran digests on gel along with 1kb and 100kb molecular ladders.
Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.

Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.

Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found

@@ Line 8: / Line 8: @@
 ** Ran digests on gel along with 1kb and 100kb molecular ladders.
 * Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
-[[Image:CIT6-28gel.jpg|thumb]]
+[[Image:CIT6-28gel.jpg|thumb|Alt=An image of the test gel of our ligation product. The middle two lanes should have bands, but apparently have none. Lane 1: 100bp ladder, Lane 2: 3uL raw ligation product, Lane 3: 15uL ligation digest, Lane 4: 1kb ladder.|Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.]]
 * Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
 ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]