Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
(→12:Transformation) |
(→13:Electrophoresis) |
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | ||
+ | |||
===13:Electrophoresis=== | ===13:Electrophoresis=== | ||
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<Sample><br /> | <Sample><br /> | ||
- | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
- | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
- | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
- | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
- | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | [[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | ||
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===14:Electrophoresis=== | ===14:Electrophoresis=== |
Revision as of 12:43, 19 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
Contents |
2010/10/15
1:DNA extraction
<Member>
Hitomi
<Sample>
<Protocol>
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
<Protocol>
See Protocol 8
3:DNA extraction from gels
<Member>
Mariko
<Sample>
(After electrophoresis)
<Protocol>
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
6:PCR
<Member>
mio
<Sample>
<Protocol>
See Protocol 2
7:DNA Digestion
<Member>
Hitomi
<Sample, Materials>
- vector
- 9-7
<Protocol>
See Protocol 9
8:DNA Ligation
<Member>
Hitomi
<Sample>
<Protocol>
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
<Protocol>
See Protocol 8
12:Transformation
<Member>
nito
<Sample>
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8