Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15

From 2010.igem.org

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(11:Electrophoresis)
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<Sample><br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
*JM109
*JM109
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ]
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===13:Electrophoresis===
===13:Electrophoresis===

Revision as of 12:42, 19 October 2010


Contents

2010/10/15

1:DNA extraction

<Member>
Hitomi

<Sample>

<Protocol>
SeeProtocol 4


2:Electrophoresis

<Member>
Hitomi

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 12hr 41min.JPG


3:DNA extraction from gels

<Member>
Mariko

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4


4:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


5:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 19hr 19min.JPG


6:PCR

<Member>
mio

<Sample>

<Protocol>
See Protocol 2


7:DNA Digestion

<Member>
Hitomi

<Sample, Materials>

<Protocol>
See Protocol 9


8:DNA Ligation

<Member>
Hitomi

<Sample>

<Protocol>
See Protocol 3


9:PCR

<Member>
mio

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


10:PCR

<Member>
nito,mizuki

<Sample>

  • 9
  • vector

<Protocol>
See Protocol 2

11:Electrophoresis

<Member>
mio

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 22hr 32min.JPG

12:Transformation

<Member>
nito

<Sample>

<Protocol>
See Protocol 7

13:Electrophoresis

<Member>
nito

<Sample>

  • 1-2-3
  • 4-5
  • 6-7
  • 9-7
  • 1


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-16 03hr 24min.JPG


14:Electrophoresis

<Member>
nito

<Sample>

  • 9
  • vector


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-16 01hr 45min.JPG