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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/11
From 2010.igem.org
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===PCR=== | ===PCR=== | ||
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nito | nito | ||
| - | + | <Sample><br /> | |
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| - | <Sample> | + | |
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*9 | *9 | ||
*1+2 | *1+2 | ||
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*vector | *vector | ||
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See Protocol2 | See Protocol2 | ||
(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/) | (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/) | ||
| - | + | *Tube(temperature in annealing)<br /> | |
| - | *Tube(temperature in annealing) | + | |
| - | + | ||
:1.(60.0,50.0℃) | :1.(60.0,50.0℃) | ||
:2.(55.0℃) | :2.(55.0℃) | ||
:3.(55.0℃) | :3.(55.0℃) | ||
:4.(55.0℃) | :4.(55.0℃) | ||
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===Electrophoresis=== | ===Electrophoresis=== | ||
Revision as of 12:16, 19 October 2010
Contents |
2010/10/11
Electrophoresis
<Member>
nito
<Sample>
veutor+7-8-10
<Protocol>
See Protocol 8
<Result>
PCR
<Member>
nito
<Sample>
- 9
- 1+2
- 10+2
- 2
- vector
<Protocol>
See Protocol2
(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
- Tube(temperature in annealing)
- 1.(60.0,50.0℃)
- 2.(55.0℃)
- 3.(55.0℃)
- 4.(55.0℃)
Electrophoresis
<Member> nito
<Sample>
PCR products.
- 9H
- 9L
- 1-2
- 10-2
- 2
- vector
<Protocol>
See Protocol8
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
<Result>
