Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03

From 2010.igem.org

(Difference between revisions)
(6:Transformation)
(7:DNA Digestion)
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<Sample, Materials><br />
<Sample, Materials><br />
・PCR productions
・PCR productions
-
*1
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*6
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6]
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*10
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10]
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・Digest enzyme
 
-
*Avr2
 
-
*Nhe1
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]

Revision as of 11:54, 19 October 2010


Contents

2010/10/03

1:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2

2:DNA extraction

<Member>
mio

<Sample>
PCR products

  • 4
  • 4

<Protocol>
See Protocol 4

3:Electrophoresis

<Member>
nito

<Sample>
All PCR products(10/3)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-03 17hr 43min.JPG

4:DNA extraction

<Member>
mio,mizuki,nito

<Sample>
PCR products

Digestion products

<Protocol>
See Protocol 4

5:DNA Ligation

<Member>
mio,mizuki

<Sample>

(after digestion)

<Protocol>
See Protocol 10

6:Transformation

<Member>
nito

<Sample>

<Protocol>
See Protocol 7

7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions


<Protocol>
See Prptpcpl 9