Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/29

From 2010.igem.org

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(3:DNA Ligation)
(4:PCR)
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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (70)
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*6  (63)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] (63)
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*7-8(70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8](70)
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*9  (58,63,69,70,72)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (58,63,69,70,72)
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*10 (58)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] (58)
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*1-2(72)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2](72)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]<br />
But today we used Kod.
But today we used Kod.

Revision as of 11:48, 19 October 2010


Contents

2010/09/29

1:DNA extraction

<Member>
nito

<Sample>
PCR products

  • 1 (9/27)①
  • 1 (9/27)②
  • 2 (9/27)
  • 3 (9/27)
  • 4 (9/27)①
  • 4 (9/27)②
  • 5(9/27)
  • 8 (9/27)
  • 10(9/27)

Digestion products (9/27)

<Protocol>
See Protocol 4

2:Electrophoresis

<Member>
nito

<Sample>
PCR products (9/27)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-29 14hr 34min.JPG

3:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 3

4:PCR

<Member>
mio,mizuki

<Sample>(temperature in annealing:℃)

  • 5 (70)
  • 6 (63)
  • 7-8(70)
  • 9 (58,63,69,70,72)
  • 10 (58)
  • 1-2(72)

<Protocol>
See Protocol 1
But today we used Kod.