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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17
From 2010.igem.org
(Difference between revisions)
(→1:PCR) |
(→2：DNA Digestion) |
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *1 (8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (8/31) |
| - | *2 (9/6) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/6) |
| - | *4 (8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (8/31) |
| - | *5L (8/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5L] (8/26) |
| - | *7 (8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] (8/31) |
| - | *8 (8/31) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (8/31) |
・Digest enzyme | ・Digest enzyme | ||
| Line 35: | Line 35: | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
| - | |||
===3:Electrophoresis①=== | ===3:Electrophoresis①=== | ||
Revision as of 11:28, 19 October 2010
Contents |
2010/09/17
1:PCR
<Member>
mio
<Sample>
(temperature in annealing)
<Protocol>
See Protocol 1
2：DNA Digestion
＜Member＞
mizuki
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- 1,4,8 : AvrⅡ
- 2,7 : NheⅠ
- 5L : SpeⅠ
＜Protocol＞
See Prptpcpl 9
3:Electrophoresis①
<Member>
mio
<Sample>
PCR products (9/17)
- 3
- 6
- 8
<Protocol>
See Protocol 8
<Result>
4:Electrophoresis②
<Member>
mizuki
<Sample>
Digestion products (9/17)
- 1
- 2
- 4
- 5
- 7
- 8
<Protocol>
See Protocol 8
<Result>
5：DNA extraction from gels
＜Member＞
mizuki
＜Sample＞
Digestion products(9/17)
- 4
- 7
- 8
(After electrophoresis)
＜Protocol＞
See Protocol 4
6：DNA Ligation
＜Member＞
mizuki
＜Sample＞
- 7(9/17)
- 8(9/17)
(after digestion)
＜Protocol＞
See Protocol 10
