Team:Caltech/Week 1

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(Difference between revisions)
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*Transformed XL-1 Blue E. Coli with selected BioBricks
*Transformed XL-1 Blue E. Coli with selected BioBricks
**[http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
**[http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
 +
* Ordered more BioBricks:
 +
** BBa_K112012
 +
** BBa_K108001
 +
** BBa_K112806
 +
** BBa_K284001
 +
** BBa_I737020
 +
(We also wanted to order sequencing primers G00100 & G00101, but apparently those aren't sent to teams.)
==Friday 6/18==
==Friday 6/18==
*Checked transformation
*Checked transformation

Revision as of 23:06, 26 June 2010


iGEM 2010



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Contents

Monday 6/14

  • Discussed procuring items & lab space.
  • Prepared for SURF start.

Tuesday 6/15

  • Created GANTT Chart (right) detailing our work plan for the summer.
GANTT Chart diagramming our planned progress throughout the summer.
  • Detailed possible side projects, open to any member as time permits:
    • Agarase
    • pH/Environmental Response
    • Phototaxis
    • Colored plastic
    • Activa (transglutaminase)
  • Procured supplies from stock room
  • Cleaned out lab space
  • Prepared LB-amp media & plates

Wednesday 6/16

  • Procured remaining antibiotics & sample of XL1 Blue cells
  • Prepare orders for Sigma, NEB, and others
    • Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
    • NEB: Biobrick assembly kit, Ladders, polymerase
  • Prepared list of biobricks to order
    • Addl Lysis bricks
    • Agarase bricks

Thursday 6/17

  • Made stock solutions for transformation and subsequent culturing
  • Transformed XL-1 Blue E. Coli with selected BioBricks
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
  • Ordered more BioBricks:
    • BBa_K112012
    • BBa_K108001
    • BBa_K112806
    • BBa_K284001
    • BBa_I737020

(We also wanted to order sequencing primers G00100 & G00101, but apparently those aren't sent to teams.)

Friday 6/18

  • Checked transformation
  • Minipreped transformed bacteria
    • Checked the plasmid concentration in the mini-prep solution
  • Made glycerol stocks from the transformed plasmid
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