Team:Washington/Team

From 2010.igem.org

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== '''Who we are''' ==
== '''Who we are''' ==
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<gallery caption="Undergrads" widths="180px" heights="120px" perrow="3">
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<gallery caption="Undergraduate Team Members" widths="180px" heights="120px" perrow="3">
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Image:Washington_jeff_johnson.png|Jeff Johnson <br/> "Eljefe" Sauro Team Member
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Image:Washington_jeff_johnson.png|<center>Jeff Johnson</center>
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Image:chris_eiben.jpg|Chris Eiben <br/> Baker Team Member <br/> Quote?
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Image:chris_eiben.jpg|<center>Chris Eiben</center>
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Image:Washington_Huber_Tomas.jpg|Tomas Huber <br/> Baker Team Member <br/> To lazy to make a quote
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Image:Washington_Huber_Tomas.jpg|<center>Tomas Huber</center>
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Image:Washington_david_zong.jpg‎|David Zong <br/> Baker Team Member <br/> Newbie!
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Image:Washington_Ivan_Huang.jpg|<center>Ivan Huang</center>
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Image:Washington_Sean_Wu.jpg|Sean Wu <br/> Baker Team Member <br/>
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Image:Washington_Peixian.JPG‎|<center>Peixian Liu</center>
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Image:Washington_Ivan_Huang.jpg|Ivan Huang <br/> Baker Team Member<br/>
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Image:Washington_Denny_Luan.JPG|<center>Denny Luan</center>
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Image:Wu_Cindy.jpg|Cindy Wu <br/> Baker Team Member<br/>
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Image:Wu_Cindy.jpg|<center>Cindy Wu</center>
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Image:Washington_Denny_Luan.JPG|Denny Luan <br/> Baker Team Member <br/>
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Image:Washington_Sean_Wu.jpg|<center>Sean Wu</center>
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Image:Laura_Austin.jpg|Laura Austin <br/> Mougous Team Member <br/>  
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Image:Washington_david_zong.jpg‎|<center>David Zong</center>
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Image:Matthew_Harger.JPG|Matthew Harger <br/> Mougous Team Member <br/> I too am too lazy to make a quote
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Image:Laura_Austin.jpg|<center>Laura Austin</center>
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Image:me2.jpg|Matt Coyne <br/> Mougous Team Member
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Image:me2.jpg|<center>Matt Coyne</center>
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Image:DSC06208.JPG|Jesa Landis <br/> Mougous Team Member
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Image:Washington_Justin.jpg|<center>Justin De Leon</center>
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Image:Washington_Justin.jpg|Justin De Leon <br/> Mougous Team Member <br/> "If I were an enzyme, I'd be DNA helicase..."
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Image:Matthew_Harger.JPG|<center>Matthew Harger</center>
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Image:DSC06208.JPG|<center>Jesa Landis</center>
</gallery>
</gallery>
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<gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3">
<gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3">
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Image:Washington_matt_smith.png|Matt Smith <br/> Molecular and Cellular Biology
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Image:Washington_matt_smith.png|<center>Matt Smith <br/> Molecular and Cellular Biology</center>
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Image:Ingrid.jpg|Ingrid Swanson <br/> Microbiology <br/> "Don't drink the 200-proof EtOH"
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Image:Ingrid.jpg|<center>Ingrid Swanson <br/> Microbiology <br/></center>
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Image:Regbert.jpg|Rob Egbert <br/> Electrical Engineering
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Image:Regbert.jpg|<center>Rob Egbert <br/> Electrical Engineering</center>
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Image:galdzicki.jpg|Michal Galdzicki <br/> Biomedical and Health Informatics
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Image:galdzicki.jpg|<center>Michal Galdzicki <br/> Biomedical and Health Informatics</center>
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Image:Washington_matt_smith.png|Justin Siegel <br/> Biomolecular Structure and Design
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Image:Washington_Siegel_2010.jpg|<center>Justin Siegel <br/> Biomolecular Structure and Design</center>
</gallery>
</gallery>
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<gallery caption="Faculty Advisors" widths="180px" heights="120px" perrow="3">
<gallery caption="Faculty Advisors" widths="180px" heights="120px" perrow="3">
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Image:Washington_matt_smith.png|David Baker <br/> Biochemistry
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image:Washington_David_Baker.jpg|<center>David Baker <br/> Biochemistry</center>
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Image:Ingrid.jpg|Joeseph Mougous <br/> Microbiology <br/>  
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Image:Washington_Klavins.jpg|<center>Eric Klavins <br/> Electrical Engineering</center>
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Image:Regbert.jpg|Eric Klavins <br/> Electrical Engineering
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image:Washington_Mougous.jpg|<center>Joseph Mougous <br/> Microbiology</center>
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Image:galdzicki.jpg|Herbert Sauro <br/> Biomedical and Health Informatics
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Image:Washington_Sauro.jpg|<center>Herbert Sauro <br/> Bioengineering</center>
</gallery>
</gallery>
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<gallery caption="Collaborators and Support" widths="180px" heights="120px" perrow="3">
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Image:Washington_Biochemistry.gif|<center>Biochemistry <br/> Lab space</center>
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image:Washington_uwmb_header.jpg|<center>Microbiology <br/> Financial support and lab space</center>
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Image:Wahsington_Fisher_Logo.jpg|<center>Fisher <br/> Lab Materials</center>
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Image:Washington_Arthur_Friedlander.jpg|<center>Arthur Friedlander @ USAMRIID <br/> Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!</center>
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Image:Washington_Chandran.png|<center>Deepak Chandran @ Bioengineering, University of Washington<br/> Always available to answer our TinkerCell API questions no matter how trivial.</center>
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</gallery>
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== '''Who did what''' ==
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The teams were assembled during our winter quarter.  During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures.  During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out.  Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
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=== The Gram Positive Therapeutic ===
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Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.
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=== The Gram Negative Therapeutic ===
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Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.
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== '''What we did''' ==
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Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.
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(Provide proper attribution for all work)
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=== New Tools ===
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Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.
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Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen.  He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.
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== '''Where we're from''' ==
 
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'''&larr; [[Team:Washington/Safety|Safety Information]]'''
'''&larr; [[Team:Washington/Safety|Safety Information]]'''
&nbsp; &nbsp; &nbsp;
&nbsp; &nbsp; &nbsp;
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'''[[Team:Washington/Notebook|Lab Notebook]] &rarr;'''
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'''[[Team:Washington/Accomplishments|What We Accomplished]] &rarr;'''
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Latest revision as of 01:01, 28 October 2010

Who we are

Undergraduate Team Members
Jeff Johnson
Chris Eiben
Tomas Huber
Ivan Huang
Peixian Liu
Denny Luan
Cindy Wu
Sean Wu
David Zong
Laura Austin
Matt Coyne
Justin De Leon
Matthew Harger
Jesa Landis


Graduate Advisors
Matt Smith
Molecular and Cellular Biology
Ingrid Swanson
Microbiology
Rob Egbert
Electrical Engineering
Michal Galdzicki
Biomedical and Health Informatics
Justin Siegel
Biomolecular Structure and Design



Faculty Advisors
David Baker
Biochemistry
Eric Klavins
Electrical Engineering
Joseph Mougous
Microbiology
Herbert Sauro
Bioengineering


Collaborators and Support
Biochemistry
Lab space
Microbiology
Financial support and lab space
Fisher
Lab Materials
Arthur Friedlander @ USAMRIID
Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!
Deepak Chandran @ Bioengineering, University of Washington
Always available to answer our TinkerCell API questions no matter how trivial.

Who did what

The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.

The Gram Positive Therapeutic

Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.

The Gram Negative Therapeutic

Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.

Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.

New Tools

Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.

Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.


← Safety Information       What We Accomplished →

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