Team:Lethbridge/Notebook/Lab Work/July
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Ran gel at 100V for 45 minutes.<br> | Ran gel at 100V for 45 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100705AVPCR.JPG|200px]]<br> | ||
==<font color="white">July 5/2010 Evening== | ==<font color="white">July 5/2010 Evening== | ||
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Ran at 100V for 80 mins.<br> | Ran at 100V for 80 mins.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100705AV BW (2).jpg|200px]]<br> | ||
<font color ="Red">More to fill in, but Anthony does not understand the stuff written in the lab book</font><br> | <font color ="Red">More to fill in, but Anthony does not understand the stuff written in the lab book</font><br> | ||
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Ran at 100V for 40 minutes. Strained in EtBr for 10 minutes.<br> | Ran at 100V for 40 minutes. Strained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100710JV BW.JPG|200px]] | ||
==<font color="white">July 12/2010== | ==<font color="white">July 12/2010== | ||
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</table><br> | </table><br> | ||
Ran at 100V for 43 minutes. Stained in EtBr for 10 minutes.<br> | Ran at 100V for 43 minutes. Stained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100712HB REstriction.JPG|200px]]<br> | ||
4) Ligate restricted dT to the ends of the "Part 1" Biobricks.<br> | 4) Ligate restricted dT to the ends of the "Part 1" Biobricks.<br> | ||
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Ran at 100V for __ minutes. Stained in EtBr for 10 minutes.<br> | Ran at 100V for __ minutes. Stained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100713JV PCR.JPG|200px]]<br> | ||
==<font color="white">July 14/2010== | ==<font color="white">July 14/2010== | ||
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Gel ran for 70 minutes at 100V and was stained in EtBr for 10 minutes | Gel ran for 70 minutes at 100V and was stained in EtBr for 10 minutes | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100719HBAVDigest.JPG|200px]]<br> | ||
Results after quantifying restriction digest | Results after quantifying restriction digest | ||
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*Ran a 1% Agarose gel to visualize PCR products. | *Ran a 1% Agarose gel to visualize PCR products. | ||
- | <font color =" | + | <b><font color="white">Results:</font></b> |
+ | Agarose gel did not show any bands.<br> | ||
==<font color="white">July 20, 2010 Evening== | ==<font color="white">July 20, 2010 Evening== | ||
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*TetR maxiprep<br> | *TetR maxiprep<br> | ||
Gel ran at 100V for 60minutes.<br> | Gel ran at 100V for 60minutes.<br> | ||
- | <b>Results:</b> The PCR's did not work. | + | <b>Results:</b> The PCR's did not work. However, the maxipreps show DNA<br> |
+ | [[image:Lethbridge_100722 JV BW.jpg|200px]] | ||
==<font color="white">July 23, 2010== | ==<font color="white">July 23, 2010== | ||
(JV)<br> | (JV)<br> | ||
<b>Objective:</b> Insert xylE, Mms6, and lumazine synthase into pET-28(a).<br> | <b>Objective:</b> Insert xylE, Mms6, and lumazine synthase into pET-28(a).<br> | ||
- | <b>Method:</b> A restriction digest was performed on xylE and Mms6 and ran for 90 minutes at 37 C | + | <b>Method:</b> A restriction digest was performed on xylE and Mms6 and ran for 90 minutes at 37 C<br> |
+ | <b>Results:</b>Did not see any cut out biobricks.<br> | ||
+ | [[image:Lethbridge_100723MaxiprepRestrictions.jpg|200px]] | ||
==<font color="white">July 27, 2010== | ==<font color="white">July 27, 2010== | ||
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<b>Method:</b> PCR amplified pSB1C3 received from iGEM headquarters. | <b>Method:</b> PCR amplified pSB1C3 received from iGEM headquarters. | ||
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==<font color="white">July 29, 2010== | ==<font color="white">July 29, 2010== | ||
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<b>Results:</b> PCR amplification was successful and 0.2 µL of polymerase will be used per PCR reaction in the future.<br> | <b>Results:</b> PCR amplification was successful and 0.2 µL of polymerase will be used per PCR reaction in the future.<br> | ||
+ | |||
<br><br> | <br><br> | ||
<b>Objective:</b> To maxiprep EYFP (E0030), ECFP (E0020), and Lumazine Synthase (K249002).<br> | <b>Objective:</b> To maxiprep EYFP (E0030), ECFP (E0020), and Lumazine Synthase (K249002).<br> | ||
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<b>Objective:</b> Run PCR products from the morning on a 2% agarose gel.<br> | <b>Objective:</b> Run PCR products from the morning on a 2% agarose gel.<br> | ||
- | <b>Method:</b> Ran 2% agarose gel of the 11 minipreps and dT control and stained in EtBr for 17 minutes. | + | <b>Method:</b> Ran 2% agarose gel of the 11 minipreps and dT control and stained in EtBr for 17 minutes.<br> |
- | + | <b>Results:</b>PCR amplification of last year's parts not consistent with sizes listed on registry.<br> | |
- | <b>Results:</b> < | + | [[image:Lethbridge_100729 Registry Parts PCR KG.jpg|200px]]<br><br> |
+ | <br> |