Team:Washington/Team
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== '''Who we are''' == | == '''Who we are''' == | ||
+ | |||
+ | <gallery caption="Undergraduate Team Members" widths="180px" heights="120px" perrow="3"> | ||
+ | Image:Washington_jeff_johnson.png|<center>Jeff Johnson</center> | ||
+ | Image:chris_eiben.jpg|<center>Chris Eiben</center> | ||
+ | Image:Washington_Huber_Tomas.jpg|<center>Tomas Huber</center> | ||
+ | Image:Washington_Ivan_Huang.jpg|<center>Ivan Huang</center> | ||
+ | Image:Washington_Peixian.JPG|<center>Peixian Liu</center> | ||
+ | Image:Washington_Denny_Luan.JPG|<center>Denny Luan</center> | ||
+ | Image:Wu_Cindy.jpg|<center>Cindy Wu</center> | ||
+ | Image:Washington_Sean_Wu.jpg|<center>Sean Wu</center> | ||
+ | Image:Washington_david_zong.jpg|<center>David Zong</center> | ||
+ | Image:Laura_Austin.jpg|<center>Laura Austin</center> | ||
+ | Image:me2.jpg|<center>Matt Coyne</center> | ||
+ | Image:Washington_Justin.jpg|<center>Justin De Leon</center> | ||
+ | Image:Matthew_Harger.JPG|<center>Matthew Harger</center> | ||
+ | Image:DSC06208.JPG|<center>Jesa Landis</center> | ||
+ | </gallery> | ||
+ | |||
+ | |||
+ | |||
<gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3"> | <gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3"> | ||
- | Image:Washington_matt_smith.png|Matt Smith | + | Image:Washington_matt_smith.png|<center>Matt Smith <br/> Molecular and Cellular Biology</center> |
- | Image:Ingrid.jpg|Ingrid Swanson <br/> Microbiology <br/> | + | Image:Ingrid.jpg|<center>Ingrid Swanson <br/> Microbiology <br/></center> |
- | Image:Regbert.jpg|Rob Egbert <br/> Electrical Engineering | + | Image:Regbert.jpg|<center>Rob Egbert <br/> Electrical Engineering</center> |
- | Image:galdzicki.jpg|Michal Galdzicki <br/> Biomedical and Health Informatics | + | Image:galdzicki.jpg|<center>Michal Galdzicki <br/> Biomedical and Health Informatics</center> |
+ | Image:Washington_Siegel_2010.jpg|<center>Justin Siegel <br/> Biomolecular Structure and Design</center> | ||
</gallery> | </gallery> | ||
- | <gallery caption=" | + | |
- | + | ||
- | + | <gallery caption="Faculty Advisors" widths="180px" heights="120px" perrow="3"> | |
- | Image: | + | image:Washington_David_Baker.jpg|<center>David Baker <br/> Biochemistry</center> |
- | + | Image:Washington_Klavins.jpg|<center>Eric Klavins <br/> Electrical Engineering</center> | |
- | Image: | + | image:Washington_Mougous.jpg|<center>Joseph Mougous <br/> Microbiology</center> |
+ | Image:Washington_Sauro.jpg|<center>Herbert Sauro <br/> Bioengineering</center> | ||
</gallery> | </gallery> | ||
- | |||
- | ( | + | <gallery caption="Collaborators and Support" widths="180px" heights="120px" perrow="3"> |
+ | Image:Washington_Biochemistry.gif|<center>Biochemistry <br/> Lab space</center> | ||
+ | image:Washington_uwmb_header.jpg|<center>Microbiology <br/> Financial support and lab space</center> | ||
+ | Image:Wahsington_Fisher_Logo.jpg|<center>Fisher <br/> Lab Materials</center> | ||
+ | Image:Washington_Arthur_Friedlander.jpg|<center>Arthur Friedlander @ USAMRIID <br/> Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!</center> | ||
+ | Image:Washington_Chandran.png|<center>Deepak Chandran @ Bioengineering, University of Washington<br/> Always available to answer our TinkerCell API questions no matter how trivial.</center> | ||
+ | </gallery> | ||
+ | |||
+ | == '''Who did what''' == | ||
+ | The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time. | ||
+ | |||
+ | === The Gram Positive Therapeutic === | ||
+ | Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants. | ||
+ | |||
+ | === The Gram Negative Therapeutic === | ||
+ | Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts. | ||
+ | |||
+ | Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid. | ||
+ | |||
+ | === New Tools === | ||
+ | Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document. | ||
+ | Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks. | ||
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<!---------------------------------------PAGE CONTENT GOES ABOVE THIS----------------------------------------> | <!---------------------------------------PAGE CONTENT GOES ABOVE THIS----------------------------------------> | ||
<div style="text-align:center"> | <div style="text-align:center"> | ||
- | '''← [[Team:Washington | + | '''← [[Team:Washington/Safety|Safety Information]]''' |
| | ||
- | '''[[Team:Washington/ | + | '''[[Team:Washington/Accomplishments|What We Accomplished]] →''' |
</div> | </div> | ||
{{Template:Team:Washington/Templates/Footer}} | {{Template:Team:Washington/Templates/Footer}} |
Latest revision as of 01:01, 28 October 2010
Who we are
Who did what
The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
The Gram Positive Therapeutic
Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.
The Gram Negative Therapeutic
Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.
Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.
New Tools
Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.
Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.