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Team:Newcastle/Meetings/18 August 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | =18th August | + | ==Formal Meeting - 18th August 2010== |
| - | ==Roll call== | + | *[[Team:Newcastle/Agendas/18_August_2010| Agenda]] |
| + | ====Roll call==== | ||
Apologies: Wendy, Colin Davie, Colin Harwood | Apologies: Wendy, Colin Davie, Colin Harwood | ||
| - | ==Action points from last meeting== | + | ====Action points from last meeting==== |
* Natto has been ordered by Wendy. | * Natto has been ordered by Wendy. | ||
| - | * Judging criteria, not | + | * Judging criteria, not available yet. |
* Details of most bricks have been entered into the parts registry, some issues with overlapping coding sequences at the moment. Will enter the problem DNA as translational units instead. | * Details of most bricks have been entered into the parts registry, some issues with overlapping coding sequences at the moment. Will enter the problem DNA as translational units instead. | ||
* iGEM logo has been added. | * iGEM logo has been added. | ||
| Line 14: | Line 15: | ||
* Rough presentation slides have been prepared. | * Rough presentation slides have been prepared. | ||
| - | ==Wiki== | + | ====Wiki==== |
| - | Up-to-date | + | Up-to-date |
| - | ==Lab feedback== | + | ====Lab feedback==== |
* Problems with ''rocF'' BioBrick (the prefix and suffix anneal back together on the plasmid, and some undigested vector was accidentally gel extracted). We have redesigned the primers and digested the plasmid template for next time. | * Problems with ''rocF'' BioBrick (the prefix and suffix anneal back together on the plasmid, and some undigested vector was accidentally gel extracted). We have redesigned the primers and digested the plasmid template for next time. | ||
* Same problem with subtilin immunity. | * Same problem with subtilin immunity. | ||
| Line 23: | Line 24: | ||
* ''yneA'' needs to be sent to the parts registry in pSB1C3. | * ''yneA'' needs to be sent to the parts registry in pSB1C3. | ||
| - | ==Modelling== | + | ====Modelling==== |
Will be our focus after August. | Will be our focus after August. | ||
| - | ==Presentation== | + | ====Presentation==== |
* May want to change the ordering of the slides around based on how well they go. | * May want to change the ordering of the slides around based on how well they go. | ||
| - | |||
* Need to show us sticking concrete together with ''Bacillus'' somewhere. | * Need to show us sticking concrete together with ''Bacillus'' somewhere. | ||
* Re-engineer based upon our outline. | * Re-engineer based upon our outline. | ||
| + | * Emphasise we are doing over expression, antisense repression, and transfer of genes from another species. And we are also doing civil engineering testing. | ||
| + | * Watched Groningen's 2009 presentation. | ||
| - | ==Action points== | + | ====Action points==== |
# Phil: Neil wants us to write next to each of the medal criteria how we will achieve it. Could characterise the AND-gate or last year's repressor system if hyperspankoid fails. Or help another team? Gibson software tool? Anyone not modelling? Anyone using ''Bacillus subtilis''? Gibson positive control (plasmid that comes back together and expresses GFP)? See judging form. | # Phil: Neil wants us to write next to each of the medal criteria how we will achieve it. Could characterise the AND-gate or last year's repressor system if hyperspankoid fails. Or help another team? Gibson software tool? Anyone not modelling? Anyone using ''Bacillus subtilis''? Gibson positive control (plasmid that comes back together and expresses GFP)? See judging form. | ||
# Can we transform ''Bacillus sphaericus''? Someone in the building has a method for transforming environmental ''Bacillus''. Want to show the calcium carbonate/glue/filamentous cell matrix. | # Can we transform ''Bacillus sphaericus''? Someone in the building has a method for transforming environmental ''Bacillus''. Want to show the calcium carbonate/glue/filamentous cell matrix. | ||
| - | # Have to present on the 15th October. | + | # Have to present on the 15th October, and also for Chris Baldwin/Biomedical school. |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 01:26, 26 October 2010
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Contents |
Formal Meeting - 18th August 2010
Roll call
Apologies: Wendy, Colin Davie, Colin Harwood
Action points from last meeting
- Natto has been ordered by Wendy.
- Judging criteria, not available yet.
- Details of most bricks have been entered into the parts registry, some issues with overlapping coding sequences at the moment. Will enter the problem DNA as translational units instead.
- iGEM logo has been added.
- Names have been sent to Janetta.
- Rough presentation slides have been prepared.
Wiki
Up-to-date
Lab feedback
- Problems with rocF BioBrick (the prefix and suffix anneal back together on the plasmid, and some undigested vector was accidentally gel extracted). We have redesigned the primers and digested the plasmid template for next time.
- Same problem with subtilin immunity.
- yneA has arrived. Will have to characterise this and its hyperspankoid promoter.
- yneA needs to be sent to the parts registry in pSB1C3.
Modelling
Will be our focus after August.
Presentation
- May want to change the ordering of the slides around based on how well they go.
- Need to show us sticking concrete together with Bacillus somewhere.
- Re-engineer based upon our outline.
- Emphasise we are doing over expression, antisense repression, and transfer of genes from another species. And we are also doing civil engineering testing.
- Watched Groningen's 2009 presentation.
Action points
- Phil: Neil wants us to write next to each of the medal criteria how we will achieve it. Could characterise the AND-gate or last year's repressor system if hyperspankoid fails. Or help another team? Gibson software tool? Anyone not modelling? Anyone using Bacillus subtilis? Gibson positive control (plasmid that comes back together and expresses GFP)? See judging form.
- Can we transform Bacillus sphaericus? Someone in the building has a method for transforming environmental Bacillus. Want to show the calcium carbonate/glue/filamentous cell matrix.
- Have to present on the 15th October, and also for Chris Baldwin/Biomedical school.
   
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