Latest revision as of 10:26, 13 August 2010

NanoDrop Spectrophotometer

Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.

Log onto computer and select Nanodrop program from the desktop (ND 1000)
Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
Use DNA-50 for DNA samples.
Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.

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@@ Line 18: / Line 18: @@
 # Log onto computer and select Nanodrop program from the desktop (ND 1000)
-# Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
+# Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.[[Image:drop3.jpg|150px]]  [[Image:drop.jpg|150px]]  [[Image:drop2.jpg|150px]]
-[[Image:drop.jpg|350px]][[Image:drop2.jpg|350px]]
+# After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
-# After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer).
 # Use DNA-50 for DNA samples.
 # Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.