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Team:Newcastle/Meetings/26 July 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | == | + | ==Formal Meeting - 26th July 2010== |
| - | + | ||
| - | ==Research feedback== | + | *[[Team:Newcastle/Agendas/26_July_2010| Agenda]] |
| + | ====Roll call==== | ||
| + | *Everyone present except Da, Prof. Colin Harwood, Dr Colin Davie and Dr Wendy Smith | ||
| + | |||
| + | ====Research feedback==== | ||
# Levan glue - Levan requires sucrose to work | # Levan glue - Levan requires sucrose to work | ||
| - | * Test how much sucrose is needed - Phil | + | #* Test how much sucrose is needed - Phil |
# Kill switch - Use of ''mazE'' and ''mazF'' | # Kill switch - Use of ''mazE'' and ''mazF'' | ||
| - | * How much sucrose is needed for it to work? | + | #* How much sucrose is needed for it to work? |
| - | * How fast does the bug use up all the sucrose? | + | #* How fast does the bug use up all the sucrose? |
# Stochastic switch - Use of ''hinC'' as the invertase | # Stochastic switch - Use of ''hinC'' as the invertase | ||
| - | * Matt to work with Phil to work out the probability of the switching | + | #* Matt to work with Phil to work out the probability of the switching |
| - | * Have to work out what is wrong with the iGEM 2009 stochastic switch first | + | #* Have to work out what is wrong with the iGEM 2009 stochastic switch first |
# Gibson protocol | # Gibson protocol | ||
| - | * ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | + | #* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first |
| + | # Protocol for ''yneA'' | ||
| + | #* Do up the characterization separately, one bacteria to contain ''yneA'' to show filamentous, the other to have the ''lacI'' | ||
| + | #* Could have nice photo with the different morphology on agar | ||
| + | #* Do not shine UV light on during the gel extraction step to avoid DNA mutation | ||
| + | #* Make up lots of DNA to allow better transformation | ||
| - | ==Lab feedback== | + | ====Lab feedback==== |
# Arginine test - Alan and Harsh | # Arginine test - Alan and Harsh | ||
| - | * Test worked fine. | + | #* Test worked fine. |
| - | * Could refine the test by using universal liquid pH indicator or to use the pH meter | + | #* Could refine the test by using universal liquid pH indicator or to use the pH meter |
# DNA extraction - Harsh and Racheal | # DNA extraction - Harsh and Racheal | ||
| - | * Could only confirm the result on 27.07.2010 | + | #* Could only confirm the result on 27.07.2010 |
| - | ==Wiki== | + | ====Wiki==== |
| - | * Not able to do the drop down | + | * Not able to do the drop down menu with the shadow test, therefore could try using htm5 |
| - | * Horizontal strip to change to purple | + | * Horizontal strip to change to purple |
| - | ==Visas== | + | ====Visas==== |
| - | * Deena to travel to London on 12.08.2010 | + | * Deena to travel to London on 12.08.2010 |
| - | ==Action point== | + | ====Action point==== |
| - | * Talk to | + | * Talk to Louela - Phil and Harsh |
* Email to iGEM HQ to get stickers and badges - Harsh | * Email to iGEM HQ to get stickers and badges - Harsh | ||
* Hyperlink the protocol page to the lab notebook | * Hyperlink the protocol page to the lab notebook | ||
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* Remind Neil about the MrGene logo - Steven | * Remind Neil about the MrGene logo - Steven | ||
| - | ==Next meeting== | + | ====Next meeting==== |
| - | * Phil | + | * Chair: Phil, Computer: Alan, Minutes: Rachel |
| - | * Wednesday | + | * Next Wednesday, 3pm |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 01:36, 26 October 2010
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Contents |
Formal Meeting - 26th July 2010
Roll call
- Everyone present except Da, Prof. Colin Harwood, Dr Colin Davie and Dr Wendy Smith
Research feedback
- Levan glue - Levan requires sucrose to work
- Test how much sucrose is needed - Phil
- Kill switch - Use of mazE and mazF
- How much sucrose is needed for it to work?
- How fast does the bug use up all the sucrose?
- Stochastic switch - Use of hinC as the invertase
- Matt to work with Phil to work out the probability of the switching
- Have to work out what is wrong with the iGEM 2009 stochastic switch first
- Gibson protocol
- rocF to require 6 pair of primers, therefore try doing up 2 fragments first
- Protocol for yneA
- Do up the characterization separately, one bacteria to contain yneA to show filamentous, the other to have the lacI
- Could have nice photo with the different morphology on agar
- Do not shine UV light on during the gel extraction step to avoid DNA mutation
- Make up lots of DNA to allow better transformation
Lab feedback
- Arginine test - Alan and Harsh
- Test worked fine.
- Could refine the test by using universal liquid pH indicator or to use the pH meter
- DNA extraction - Harsh and Racheal
- Could only confirm the result on 27.07.2010
Wiki
- Not able to do the drop down menu with the shadow test, therefore could try using htm5
- Horizontal strip to change to purple
Visas
- Deena to travel to London on 12.08.2010
Action point
- Talk to Louela - Phil and Harsh
- Email to iGEM HQ to get stickers and badges - Harsh
- Hyperlink the protocol page to the lab notebook
- Lab wiki checker - Alan and Harsh
- Send reminder to Jen to add Jane to the mailing list - Jannetta
- Put CBCB and Biomedical school logo onto the wiki - Janetta
- Write to Chris Voigt to get the source code - Steven
- Tutorial for Inkscape - Jannetta
- To check if the extra USA visa immigration form is valid for 2 years - Jen
- Remind Neil about the MrGene logo - Steven
Next meeting
- Chair: Phil, Computer: Alan, Minutes: Rachel
- Next Wednesday, 3pm
   
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